Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays
DC Field | Value | Language |
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dc.contributor.author | Chen, Jun-Hu | - |
dc.contributor.author | Wang, Yue | - |
dc.contributor.author | Ha, Kwon-Soo | - |
dc.contributor.author | Lu, Feng | - |
dc.contributor.author | Suh, In-Bum | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.contributor.author | Park, Jeong Hyun | - |
dc.contributor.author | Takeo, Satoru | - |
dc.contributor.author | Tsuboi, Takafumi | - |
dc.contributor.author | Han, Eun-Taek | - |
dc.date.accessioned | 2021-09-07T06:48:07Z | - |
dc.date.available | 2021-09-07T06:48:07Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 0932-0113 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111303 | - |
dc.description.abstract | Protein arrays are powerful tools for antibody profiling and vaccine development against infectious agents. In the previous report, we successfully applied an antibody-based protein array for immunoprofiling of Plasmodium vivax infection. Herein, we developed a Ni-NTA surface based protein array to detect immune responses against the recombinant C-terminal region (19 and 42 kDa) of the P. vivax merozoite surface protein 1 (PvMSP1-19 and -42) from sera of vivax malaria patients. The PvMSP1-19 arrays detected P. vivax in 112 of 130 (86.2%; 95% CI, 83.2-89.2%) microscopically positive samples and 2 false positives were obtained among 100 sera samples from healthy subjects (2.0%; 95% CI, 0.6-3.4%). These results were in concordance with results of enzyme-linked immunosorbent assays (ELISA). Kappa values represented excellent agreement for the recombinant PvMSP1-19 protein against sera samples as measured by protein arrays and ELISA (Kappa = 0.904, 95% CI: 0.849-0.960). The PvMSP1-42 protein arrays detected antibody response in 100 of 130 microscopically positive samples (76.9%; 95% CI, 72.4-86.8%) and 8 false positives were obtained in 100 healthy subjects (8.0%; 95% CI, 2.7-13.3%). There is no significant difference between the fluorescent intensity of antibody response to PvMSP1-19 and PvMSP1-42 in the positive sera samples (P > 0.05). The novel protein array platform may be used for profiling naturally acquired humoral immune responses to P. vivax infection. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | MEROZOITE SURFACE PROTEIN-1 | - |
dc.subject | HUMAN MALARIA PARASITE | - |
dc.subject | ANTIBODY-RESPONSES | - |
dc.subject | MICROARRAY REVEALS | - |
dc.subject | CANDIDATE ANTIGENS | - |
dc.subject | VACCINE CANDIDATES | - |
dc.subject | FALCIPARUM | - |
dc.subject | IDENTIFICATION | - |
dc.subject | INFECTION | - |
dc.subject | SERODIAGNOSIS | - |
dc.title | Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Chae Seung | - |
dc.identifier.doi | 10.1007/s00436-011-2370-z | - |
dc.identifier.scopusid | 2-s2.0-82955182188 | - |
dc.identifier.wosid | 000296011300005 | - |
dc.identifier.bibliographicCitation | PARASITOLOGY RESEARCH, v.109, no.5, pp.1259 - 1266 | - |
dc.relation.isPartOf | PARASITOLOGY RESEARCH | - |
dc.citation.title | PARASITOLOGY RESEARCH | - |
dc.citation.volume | 109 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1259 | - |
dc.citation.endPage | 1266 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Parasitology | - |
dc.relation.journalWebOfScienceCategory | Parasitology | - |
dc.subject.keywordPlus | MEROZOITE SURFACE PROTEIN-1 | - |
dc.subject.keywordPlus | HUMAN MALARIA PARASITE | - |
dc.subject.keywordPlus | ANTIBODY-RESPONSES | - |
dc.subject.keywordPlus | MICROARRAY REVEALS | - |
dc.subject.keywordPlus | CANDIDATE ANTIGENS | - |
dc.subject.keywordPlus | VACCINE CANDIDATES | - |
dc.subject.keywordPlus | FALCIPARUM | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | SERODIAGNOSIS | - |
dc.subject.keywordAuthor | MSP P.vivax | - |
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