Effect of hypertonic saline and macrophage migration inhibitory factor in restoration of T cell dysfunction
DC Field | Value | Language |
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dc.contributor.author | Yoon, Young-Hoon | - |
dc.contributor.author | Choi, Sung-Hyuk | - |
dc.contributor.author | Hong, Yun-Sik | - |
dc.contributor.author | Lee, Sung-Woo | - |
dc.contributor.author | Moon, Sung-Woo | - |
dc.contributor.author | Cho, Han-Jin | - |
dc.contributor.author | Han, Cheul | - |
dc.contributor.author | Cheon, Young-Jin | - |
dc.contributor.author | Bansal, Vishal | - |
dc.date.accessioned | 2021-09-07T07:38:26Z | - |
dc.date.available | 2021-09-07T07:38:26Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 2233-7903 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111403 | - |
dc.description.abstract | Purpose: Trauma-induced suppression of cellular immune function likely contributes to sepsis, multiple organ dysfunction syndrome and death. T cell proliferation decreases after traumatic stress. The addition of prostaglandin E(2) (PGE(2)), which depresses immune function after hemorrhage and trauma, to T-cells decreases T-cell proliferation; and hypertonic saline restores PGE(2)-induced T-cell suppression. Recently, it has become apparent that macrophage migration inhibitory factor (MIF) plays a central role in several immune responses, including T-cell proliferation. However, the role of MIF in mediating hypertonic saline (HTS) restoration of T cell dysfunction is unknown. Therefore, we hypothesize that T cell immune restoration by HTS occurs, at least in part, by a MIF-mediated mechanism. Methods: Jurkat cells were cultured in Roswell Park Memorial Institute media, at a final concentration of 2.5 x 10(6) cell/mL. The effects of HTS on T-cell proliferation following PGE(2)-induced suppression were evaluated in Jurkat HTS at 20 or 40 mmol/L above isotonicity was added. MIF levels were determined by enzyme-linked immunosorbent assay and western blot analysis. Results: PGE2 caused a 15.0% inhibition of Jurkat cell proliferation, as compared to the control. MIF levels decreased in PGE(2)-suppressed cells, as compared to the control. MIF levels were higher in cells treated with HTS than PGE(2)-stimulated cells. Conclusion: The role of HTS in restoring Jurkat cells proliferation suppressed by PGE(2), at least in part, should be mediated through a MIF pathway. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SURGICAL SOCIETY | - |
dc.subject | FACTOR MIF | - |
dc.subject | IMMUNE-RESPONSE | - |
dc.subject | REGULATORY ROLE | - |
dc.subject | SUPPRESSION | - |
dc.subject | EXPRESSION | - |
dc.subject | TRAUMA | - |
dc.title | Effect of hypertonic saline and macrophage migration inhibitory factor in restoration of T cell dysfunction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoon, Young-Hoon | - |
dc.contributor.affiliatedAuthor | Choi, Sung-Hyuk | - |
dc.contributor.affiliatedAuthor | Hong, Yun-Sik | - |
dc.contributor.affiliatedAuthor | Lee, Sung-Woo | - |
dc.contributor.affiliatedAuthor | Moon, Sung-Woo | - |
dc.contributor.affiliatedAuthor | Cho, Han-Jin | - |
dc.identifier.doi | 10.4174/jkss.2011.81.4.229 | - |
dc.identifier.scopusid | 2-s2.0-81055137614 | - |
dc.identifier.wosid | 000295424000001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE KOREAN SURGICAL SOCIETY, v.81, no.4, pp.229 - 234 | - |
dc.relation.isPartOf | JOURNAL OF THE KOREAN SURGICAL SOCIETY | - |
dc.citation.title | JOURNAL OF THE KOREAN SURGICAL SOCIETY | - |
dc.citation.volume | 81 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 229 | - |
dc.citation.endPage | 234 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001587914 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | FACTOR MIF | - |
dc.subject.keywordPlus | IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | REGULATORY ROLE | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | TRAUMA | - |
dc.subject.keywordAuthor | Hypertonic solutions | - |
dc.subject.keywordAuthor | Macrophage Migration-Inhibitory factors | - |
dc.subject.keywordAuthor | Prostaglandins E | - |
dc.subject.keywordAuthor | Injuries | - |
dc.subject.keywordAuthor | T-lymphocytes | - |
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