The generation of iPS cells using non-viral magnetic nanoparticle based transfection
DC Field | Value | Language |
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dc.contributor.author | Lee, Chang Hyun | - |
dc.contributor.author | Kim, Jung-Hyun | - |
dc.contributor.author | Lee, Hyun Joo | - |
dc.contributor.author | Jeon, Kilsoo | - |
dc.contributor.author | Lim, HyeJin | - |
dc.contributor.author | Choi, Hye Yeon | - |
dc.contributor.author | Lee, Eung-Ryoung | - |
dc.contributor.author | Park, Seung Hwa | - |
dc.contributor.author | Park, Jae-Yong | - |
dc.contributor.author | Hong, Sunghoi | - |
dc.contributor.author | Kim, Soonhag | - |
dc.contributor.author | Cho, Ssang-Goo | - |
dc.date.accessioned | 2021-09-07T07:44:12Z | - |
dc.date.available | 2021-09-07T07:44:12Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111437 | - |
dc.description.abstract | Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transfection method that employs biodegradable cationic polymer PEI-coated super paramagnetic nanoparticles (NP). Our findings support the possible use of transient expression of iPS genes in somatic cells by magnet-based nanofection for efficient generation of iPS cells. Results of dynamic light scattering (DLS) analysis and TEM analyses demonstrated efficient conjugation of NP with iPS genes. After transfection, nanofection-mediated iPS cells showed ES cell-like characteristics, including expression of endogenous pluripotency genes, differentiation of three germ layer lineages, and formation of teratomas. Our results demonstrate that magnet-based nanofection may provide a safe method for use in generation of virus-free and exogenous DNA-free iPS cells, which will be crucial for future clinical applications in the field of regenerative medicine. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | PLURIPOTENT STEM-CELLS | - |
dc.subject | SOMATIC-CELLS | - |
dc.subject | GENE DELIVERY | - |
dc.subject | FIBROBLASTS | - |
dc.subject | MOUSE | - |
dc.subject | MAGNETOFECTION | - |
dc.subject | TRANSPOSITION | - |
dc.subject | INDUCTION | - |
dc.subject | PIGGYBAC | - |
dc.subject | FORCE | - |
dc.title | The generation of iPS cells using non-viral magnetic nanoparticle based transfection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Sunghoi | - |
dc.identifier.doi | 10.1016/j.biomaterials.2011.05.070 | - |
dc.identifier.scopusid | 2-s2.0-79960564360 | - |
dc.identifier.wosid | 000293941400005 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.32, no.28, pp.6683 - 6691 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 32 | - |
dc.citation.number | 28 | - |
dc.citation.startPage | 6683 | - |
dc.citation.endPage | 6691 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | FIBROBLASTS | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | MAGNETOFECTION | - |
dc.subject.keywordPlus | TRANSPOSITION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | PIGGYBAC | - |
dc.subject.keywordPlus | FORCE | - |
dc.subject.keywordAuthor | Induced pluripotent stem cells | - |
dc.subject.keywordAuthor | Super paramagnetic nanoparticles | - |
dc.subject.keywordAuthor | Non-viral transfection | - |
dc.subject.keywordAuthor | Embryonic stem cells | - |
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