Ago2/miRISC-mediated inhibition of CBP80/20-dependent translation and thereby abrogation of nonsense-mediated mRNA decay require the cap-associating activity of Ago2
DC Field | Value | Language |
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dc.contributor.author | Choe, Junho | - |
dc.contributor.author | Cho, Hana | - |
dc.contributor.author | Chi, Sung-Gil | - |
dc.contributor.author | Kim, Yoon Ki | - |
dc.date.accessioned | 2021-09-07T08:35:47Z | - |
dc.date.available | 2021-09-07T08:35:47Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-09-02 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111602 | - |
dc.description.abstract | Nuclear cap-binding protein (CBP) 80/20-dependent translation (CT) is one of the targets for miRNA-mediated gene silencing. Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is tightly coupled to CT. Tethering of Ago2, but not of Ago2F2V2 which lacks cap -association activity, to the 3'UTR of PTC-containing mRNA abrogates NMD. Immunoprecipitation using CBP80 antibody reveals that Ago2, but not Ago2F2V2, inhibits the binding of CBP80/20 to cap structure. Our observations provide molecular insight into the cross-talk between miRNA-mediated gene silencing, CT, and NMD. Structured summary of protein interactions: AGO2 physically interacts with GW182 by anti tag coimmunoprecipitation (View interaction) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | PROCESSING BODIES | - |
dc.subject | COMPLEX | - |
dc.subject | GW182 | - |
dc.subject | DEGRADATION | - |
dc.subject | ARGONAUTE | - |
dc.subject | DOMAIN | - |
dc.subject | SURVEILLANCE | - |
dc.subject | REPRESSION | - |
dc.subject | MIRNAS | - |
dc.title | Ago2/miRISC-mediated inhibition of CBP80/20-dependent translation and thereby abrogation of nonsense-mediated mRNA decay require the cap-associating activity of Ago2 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chi, Sung-Gil | - |
dc.contributor.affiliatedAuthor | Kim, Yoon Ki | - |
dc.identifier.doi | 10.1016/j.febslet.2011.07.047 | - |
dc.identifier.scopusid | 2-s2.0-80052260003 | - |
dc.identifier.wosid | 000294295400010 | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, v.585, no.17, pp.2682 - 2687 | - |
dc.relation.isPartOf | FEBS LETTERS | - |
dc.citation.title | FEBS LETTERS | - |
dc.citation.volume | 585 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 2682 | - |
dc.citation.endPage | 2687 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | PROCESSING BODIES | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | GW182 | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | ARGONAUTE | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | SURVEILLANCE | - |
dc.subject.keywordPlus | REPRESSION | - |
dc.subject.keywordPlus | MIRNAS | - |
dc.subject.keywordAuthor | Ago2 | - |
dc.subject.keywordAuthor | MicroRNA | - |
dc.subject.keywordAuthor | NMD | - |
dc.subject.keywordAuthor | CBP80/20 | - |
dc.subject.keywordAuthor | eIF4E | - |
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