Axin1 Expression Facilitates Cell Death Induced by Aurora Kinase Inhibition Through PARP Activation
DC Field | Value | Language |
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dc.contributor.author | Choi, Eun-Jin | - |
dc.contributor.author | Kim, Shi-Mun | - |
dc.contributor.author | Song, Ki-Joon | - |
dc.contributor.author | Lee, Jae-Myun | - |
dc.contributor.author | Kee, Sun-Ho | - |
dc.date.accessioned | 2021-09-07T08:42:54Z | - |
dc.date.available | 2021-09-07T08:42:54Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111643 | - |
dc.description.abstract | Axin, a negative regulator of Wnt signaling, participates in apoptosis, and Axin1 localizes to centrosomes and mitotic spindles, which requires Aurora kinase activity. In this study, Aurora inhibition of Axin1-expressing cells (L-Axin) produced polyploid cells, which died within 48 h posttreatment, whereas Axin2-expressing cells (L-Axin2) survived the same period. These cell death events showed apoptotic signs, such as chromatin condensation and increased sub-G1 populations, as well as cell membrane rupture. Further analysis showed that Aurora kinase inhibitor (AKI) treatment of L-Axin cells induced poly(ADP-ribose) polymerase (PARP) activation, which increased the poly(ADP-ribosyl) ation of cellular proteins and reduced cellular ATP content. PARP inhibition reduced a proportion of dead cells, suggesting PARP involvement in AKI-induced cell death. Also, AKI treatment of L-Axin cells induced mitochondrial apoptosis-inducing factor (AIF) release, but not mitochondrial cytochrome c release or caspase-3 activation. Knockdown of AIF attenuated AKI-induced cell death in L-Axin cells. Thus, our results suggest that Axin1 expression renders L929 cells sensitive to Aurora inhibition-induced cell death in a PARP-and AIF-dependent manner. J. Cell. Biochem. 112: 2392-2402, 2011. (C) 2011 Wiley-Liss, Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | SMALL-MOLECULE INHIBITOR | - |
dc.subject | BETA-CATENIN | - |
dc.subject | B KINASE | - |
dc.subject | POLY(ADP-RIBOSE) POLYMERASE-1 | - |
dc.subject | CHROMOSOMAL INSTABILITY | - |
dc.subject | UP-REGULATION | - |
dc.subject | DRUG TARGET | - |
dc.subject | IN-VIVO | - |
dc.subject | APOPTOSIS | - |
dc.subject | OVEREXPRESSION | - |
dc.title | Axin1 Expression Facilitates Cell Death Induced by Aurora Kinase Inhibition Through PARP Activation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Eun-Jin | - |
dc.contributor.affiliatedAuthor | Song, Ki-Joon | - |
dc.contributor.affiliatedAuthor | Kee, Sun-Ho | - |
dc.identifier.doi | 10.1002/jcb.23162 | - |
dc.identifier.scopusid | 2-s2.0-84860400051 | - |
dc.identifier.wosid | 000294769500022 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, v.112, no.9, pp.2392 - 2402 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.title | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.volume | 112 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2392 | - |
dc.citation.endPage | 2402 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | SMALL-MOLECULE INHIBITOR | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | B KINASE | - |
dc.subject.keywordPlus | POLY(ADP-RIBOSE) POLYMERASE-1 | - |
dc.subject.keywordPlus | CHROMOSOMAL INSTABILITY | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | DRUG TARGET | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordAuthor | AXIN | - |
dc.subject.keywordAuthor | AURORA KINASE | - |
dc.subject.keywordAuthor | CELL DEATH | - |
dc.subject.keywordAuthor | PARP | - |
dc.subject.keywordAuthor | AIF | - |
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