Molecular mechanism of HIF-1-independent VEGF expression in a hepatocellular carcinoma cell line
DC Field | Value | Language |
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dc.contributor.author | Choi, Sae Byeol | - |
dc.contributor.author | Park, Jung Bae | - |
dc.contributor.author | Song, Tae-Jin | - |
dc.contributor.author | Choi, Sang Yong | - |
dc.date.accessioned | 2021-09-07T08:58:08Z | - |
dc.date.available | 2021-09-07T08:58:08Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111699 | - |
dc.description.abstract | Hypoxia-inducible factor-1 (HIF-1) is a master transcription factor that plays a central role in the hypoxic expression of various genes. Vascular endothelial growth factor (VEGF), a known target gene of HIF-1 alpha, has been shown to be induced by hypoxia through a HIF-1 alpha-independent pathway. HIF-1 alpha dominant-negative lentiviral vectors were introduced to decrease the expression of HIF in Hep3B cells. Cells were incubated under normoxic or hypoxic conditions. We performed a VEGF enzyme-linked immunosorbent assay (ELISA) using cell culture supernatants, and Western blotting using cell lysates. To validate signaling via HIFI-dependent or HIF-1-independent pathways, we treated cells with an extracellular signal-regulated kinase (ERK) kinase inhibitor, a phosphoinositide 3-kinase (PI3K) inhibitor, and transfected cells with siSP1. HIF-1 alpha protein expression was induced and the levels of VEGF increased under hypoxic conditions. Cells were transfected with siHIF-1 alpha and incubated under normoxic or hypoxic conditions. We found that a significant amount of VEGF was produced by a HIF-1-independent pathway. PI3K inhibitor treatment and siSP1 transient transfection decreased VEGF expression in siHIF-1 alpha-transfected cells. Therefore, VEGF regulation in Hep3B cells is primarily controlled by the Akt/PI3K and SP1 pathways and is independent of HIF-1 under hypoxic conditions. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | HYPOXIA-INDUCIBLE FACTORS | - |
dc.subject | PROSTATE-CANCER CELLS | - |
dc.subject | GENE-TRANSCRIPTION | - |
dc.subject | TUMOR-FORMATION | - |
dc.subject | SP1 ACTIVITY | - |
dc.subject | KINASE-B | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | HIF-1-ALPHA | - |
dc.subject | ACTIVATION | - |
dc.title | Molecular mechanism of HIF-1-independent VEGF expression in a hepatocellular carcinoma cell line | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Sae Byeol | - |
dc.contributor.affiliatedAuthor | Song, Tae-Jin | - |
dc.contributor.affiliatedAuthor | Choi, Sang Yong | - |
dc.identifier.doi | 10.3892/ijmm.2011.719 | - |
dc.identifier.scopusid | 2-s2.0-79959968571 | - |
dc.identifier.wosid | 000293603100021 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.28, no.3, pp.449 - 454 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.volume | 28 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 449 | - |
dc.citation.endPage | 454 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | HYPOXIA-INDUCIBLE FACTORS | - |
dc.subject.keywordPlus | PROSTATE-CANCER CELLS | - |
dc.subject.keywordPlus | GENE-TRANSCRIPTION | - |
dc.subject.keywordPlus | TUMOR-FORMATION | - |
dc.subject.keywordPlus | SP1 ACTIVITY | - |
dc.subject.keywordPlus | KINASE-B | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | HIF-1-ALPHA | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | hepatocellular carcinoma | - |
dc.subject.keywordAuthor | Hep3B cell line | - |
dc.subject.keywordAuthor | angiogenesis | - |
dc.subject.keywordAuthor | vascular endothelial growth factor | - |
dc.subject.keywordAuthor | hypoxia-inducible factor-1 | - |
dc.subject.keywordAuthor | SP1 | - |
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