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Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray

Authors
Yoon, Hyun KyuChung, In HyukKim, Hee-JinLee, Ki-OKim, Jun SubPark, Eui-JinSong, Sang YongBang, Sa IkKim, Tae SungHwang, Seung Yong
Issue Date
9월-2011
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Keywords
HLA; HLA-C genotyping; Microarray; DNA chip; Transplantation
Citation
BIOCHIP JOURNAL, v.5, no.3, pp.255 - 264
Indexed
SCIE
SCOPUS
KCI
OTHER
Journal Title
BIOCHIP JOURNAL
Volume
5
Number
3
Start Page
255
End Page
264
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/111714
DOI
10.1007/s13206-011-5310-1
ISSN
1976-0280
Abstract
Human Leukocyte Antigen (HLA), called human Major Histocompatibility Complex (MHC), performs the crucial function of antigen presentation in human immunity, and correctly matched HLA antigens improves survival and reduces immunological complications. Therefore, a precise HLA typing method is necessary for successful transplantation between donor and recipient. Until now, many methods have been developed for HLA typing, including serological and DNA-based methods. However, these previous methods have been somewhat limited due to their requirements for a large number of samples and complex experimental procedures. To resolve this problem, in this study, we developed rapid genotyping methods for HLA-C using an oligonucleotide microarray. To evaluate the performance of the HLA-C genotyping microarray, we analyzed 50 unrelated Korean individuals. The genotyping results obtained by the oligonucleotide microarray were identical with those of PCR-SBT and PCR-SSPs, at a low-resolution, 2-digit level. Therefore, this microarray method for genotyping HLA constitutes a rapid, simultaneous, and high-throughput method that can be used as an alternative to current methods.
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