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In Vivo Imaging of Near-Membrane Calcium Ions with Two-Photon Probes
- Issue Date
- 1-8월-2011
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- calcium; fluorescence; nonlinear spectroscopy; microscopy; two-photon probe
- Citation
- CHEMISTRY-AN ASIAN JOURNAL, v.6, no.8, pp.2027 - 2032
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMISTRY-AN ASIAN JOURNAL
- Volume
- 6
- Number
- 8
- Start Page
- 2027
- End Page
- 2032
- ISSN
- 1861-4728
- Abstract
- We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (K-d(TP)) of (1.9 +/- 0.2) mu m, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 mu m depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.
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