Blocking the immunosuppressive axis with small interfering RNA targeting interleukin (IL)-10 receptor enhances dendritic cell-based vaccine potency
DC Field | Value | Language |
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dc.contributor.author | Kim, J. H. | - |
dc.contributor.author | Kang, T. H. | - |
dc.contributor.author | Noh, K. H. | - |
dc.contributor.author | Bae, H. C. | - |
dc.contributor.author | Ahn, Y-H. | - |
dc.contributor.author | Lee, Y-H. | - |
dc.contributor.author | Choi, E. Y. | - |
dc.contributor.author | Chun, K-H. | - |
dc.contributor.author | Lee, S-J. | - |
dc.contributor.author | Kim, T. W. | - |
dc.date.accessioned | 2021-09-07T09:43:49Z | - |
dc.date.available | 2021-09-07T09:43:49Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 0009-9104 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/111832 | - |
dc.description.abstract | Improving dendritic cell (DC) functions is highly promising for therapeutic intervention of diverse diseases, including cancer. Immunosuppressive cytokines such as interleukin (IL)-10 produced by DCs themselves (autocrine) and other regulatory immune cells (paracrine) down-regulate functional profiles of DCs through specific cell surface receptors such as IL-10R. Here, we tried to improve DC functions using small interfering RNA (siRNA) technology to block an IL-10R-mediated immunosuppressive axis. DCs modified with siRNA targeting against IL-10R or IL-10 (DC/siIL-10R or DC/siIL-10) led to up-regulation of major histocompatibility complex (MHC) class II, CD40 co-stimulatory molecule, and IL-12 proinflammatory cytokine after lipopolysacharide (LPS) stimulation compared to DC/siGFP. Notably, the LPS-induced functional profiles of DC/siIL-10R were strongly resistant to the addition of recombinant IL-10, which mimicked paracrine IL-10. In contrast, those of DC/siIL-10 were reversed by adding exogenous IL-10. Consistently, DC/siIL-10R generated more human papilloma virus (HPV) E7-specific CD8(+) T cells and stronger anti-tumour effects against E7-expressing TC-1 tumour cells in vaccinated mice than DC/siGFP, as well as DC/siIL-10. Taken together, these results provide the groundwork for future clinical translation of siRNA-mediated strategy targeting IL-10R to enhance DC-based vaccine potency. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | IN-VIVO | - |
dc.subject | T-CELL | - |
dc.subject | IMMUNE-RESPONSES | - |
dc.subject | CANCER | - |
dc.subject | IL-10 | - |
dc.subject | ANTIGEN | - |
dc.subject | INDUCTION | - |
dc.subject | TRANSCRIPTION | - |
dc.subject | TOLERANCE | - |
dc.subject | CYTOKINE | - |
dc.title | Blocking the immunosuppressive axis with small interfering RNA targeting interleukin (IL)-10 receptor enhances dendritic cell-based vaccine potency | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, T. W. | - |
dc.identifier.doi | 10.1111/j.1365-2249.2011.04410.x | - |
dc.identifier.scopusid | 2-s2.0-79960039023 | - |
dc.identifier.wosid | 000292338200006 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL IMMUNOLOGY, v.165, no.2, pp.180 - 189 | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL IMMUNOLOGY | - |
dc.citation.title | CLINICAL AND EXPERIMENTAL IMMUNOLOGY | - |
dc.citation.volume | 165 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 180 | - |
dc.citation.endPage | 189 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | T-CELL | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | IL-10 | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | TOLERANCE | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordAuthor | dendritic cell | - |
dc.subject.keywordAuthor | IL-10 receptor | - |
dc.subject.keywordAuthor | immunosuppression | - |
dc.subject.keywordAuthor | immunotherapy | - |
dc.subject.keywordAuthor | siRNA | - |
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