A novel Escherichia coli solubility enhancer protein for fusion expression of aggregation-prone heterologous proteins
DC Field | Value | Language |
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dc.contributor.author | Song, Jong-Am | - |
dc.contributor.author | Lee, Dae-Sung | - |
dc.contributor.author | Park, Jin-Seung | - |
dc.contributor.author | Han, Kyung-Yeon | - |
dc.contributor.author | Lee, Jeewon | - |
dc.date.accessioned | 2021-09-07T10:41:24Z | - |
dc.date.available | 2021-09-07T10:41:24Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-07-10 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112000 | - |
dc.description.abstract | Through the proteome analysis of Escherichia coli BL21(DE3), we previously identified the stress-responsive protein, arsenate reductase (ArsC), that showed a high cytoplasmic solubility and a folding capacity even in the presence of stress-inducing reagents. In this study, we used ArsC as an N-terminal fusion partner to synthesize nine aggregation-prone proteins as water-soluble forms. As a result, solubility of the aggregation-prone proteins increased dramatically by the fusion of ArsC, due presumably to its tendency to facilitate the folding of target proteins. Also, we evaluated and confirmed the efficacy of ArsC-fusion expression in making the fusion-expressed target proteins have their own native function or structure. That is, the self-assembly function of human ferritin light chain, L-arginine-degrading function of arginine deiminase, and the correct secondary structure of human granulocyte colony stimulating factor were clearly observed through transmission electron microscope analysis, colorimetric enzyme activity assay, and circular dichroism, respectively. It is strongly suggested that ArsC can be in general an efficient fusion expression partner for the production of soluble and active heterologous proteins in E. coli. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | MYCOPLASMA ARGININE DEIMINASE | - |
dc.subject | COLONY-STIMULATING FACTOR | - |
dc.subject | MALTOSE-BINDING PROTEIN | - |
dc.subject | HUMAN G-CSF | - |
dc.subject | RECOMBINANT PROTEINS | - |
dc.subject | BACTERIAL | - |
dc.subject | POLYPEPTIDES | - |
dc.subject | PURIFICATION | - |
dc.subject | CHAPERONE | - |
dc.subject | PLASMIDS | - |
dc.title | A novel Escherichia coli solubility enhancer protein for fusion expression of aggregation-prone heterologous proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jeewon | - |
dc.identifier.doi | 10.1016/j.enzmictec.2011.04.013 | - |
dc.identifier.scopusid | 2-s2.0-79959883233 | - |
dc.identifier.wosid | 000293439100002 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.49, no.2, pp.124 - 130 | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 49 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 124 | - |
dc.citation.endPage | 130 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | MYCOPLASMA ARGININE DEIMINASE | - |
dc.subject.keywordPlus | COLONY-STIMULATING FACTOR | - |
dc.subject.keywordPlus | MALTOSE-BINDING PROTEIN | - |
dc.subject.keywordPlus | HUMAN G-CSF | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | BACTERIAL | - |
dc.subject.keywordPlus | POLYPEPTIDES | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CHAPERONE | - |
dc.subject.keywordPlus | PLASMIDS | - |
dc.subject.keywordAuthor | Fusion partner | - |
dc.subject.keywordAuthor | ArsC | - |
dc.subject.keywordAuthor | Folding enhancer | - |
dc.subject.keywordAuthor | Stress-resistant protein | - |
dc.subject.keywordAuthor | Escherichia coli BL21(DE3) | - |
dc.subject.keywordAuthor | Proteome | - |
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