Maltosylated polyethylenimine-based triple nanocomplexes of human papillomavirus 16L1 protein and DNA as a vaccine co-delivery system
DC Field | Value | Language |
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dc.contributor.author | Cho, Hee-Jeong | - |
dc.contributor.author | Han, Su-Eun | - |
dc.contributor.author | Im, Saewon | - |
dc.contributor.author | Lee, Young | - |
dc.contributor.author | Kim, Young Bong | - |
dc.contributor.author | Chun, Taehoon | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.date.accessioned | 2021-09-07T10:54:45Z | - |
dc.date.available | 2021-09-07T10:54:45Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-07 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112050 | - |
dc.description.abstract | To improve vaccine delivery, we herein designed a co-delivery system using a protein antigen and its encoding plasmid linked in nanocomplexes via maltosylated PEI (mPEI). Cationic mPEI was electrostatically complexed to a plasmid encoding the human papillomavirus (HPV) type 16L1 protein (pHPV16L1), and further complexed to a maltose binding protein (MBP)-fused human papillomavirus type 16L1 fusion protein (HPV16L1-MBP). The HPV16L1-MBP/mPEI/pHPV16L1 complexes were characterized by gel-retardation properties, zeta potentials and sizes. The intracellular co-delivery of protein and plasmid DNA vaccines was significantly higher for mPEI-based triple nanocomplexes than for a simple physical mixture of the proteins and DNA. Moreover, the cellular delivery of plasmid DNA using mPEI-based triple nanocomplexes resulted in higher expression levels comparable to those obtained using dual complexes of mPEI and the plasmid DNA. In vivo, co-immunization of mice with HPV16L1-MBP/mPEI/pHPV16L1 nanocomplexes triggered the highest levels of humoral immune responses among various vaccination groups. Moreover, the mPEI-based nanocomplexes significantly enhanced the number of interferon-gamma producing CD8(+) T cells compared with the use of mixed proteins and plasmid DNA. These results suggest that the effective cellular co-delivery of MBP-fused antigen proteins and plasmid DNA using maltosylated PEI-based triple nanocomplexes could enhance the immunogenicity of HPV16L1 vaccines. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | MEDIATED GENE DELIVERY | - |
dc.subject | IMMUNE-RESPONSES | - |
dc.subject | BINDING PROTEIN | - |
dc.subject | IMMUNIZATION | - |
dc.subject | COMPLEXES | - |
dc.subject | NANOPARTICLES | - |
dc.subject | EFFICIENCY | - |
dc.subject | TRANSFECTION | - |
dc.subject | STRATEGIES | - |
dc.subject | PROTECTION | - |
dc.title | Maltosylated polyethylenimine-based triple nanocomplexes of human papillomavirus 16L1 protein and DNA as a vaccine co-delivery system | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Taehoon | - |
dc.identifier.doi | 10.1016/j.biomaterials.2011.03.004 | - |
dc.identifier.scopusid | 2-s2.0-79955521051 | - |
dc.identifier.wosid | 000291193700016 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.32, no.20, pp.4621 - 4629 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 32 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 4621 | - |
dc.citation.endPage | 4629 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | MEDIATED GENE DELIVERY | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | BINDING PROTEIN | - |
dc.subject.keywordPlus | IMMUNIZATION | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordAuthor | Vaccine co-delivery systems | - |
dc.subject.keywordAuthor | Triple nanocomplexes | - |
dc.subject.keywordAuthor | Maltosylated polyethylenimine | - |
dc.subject.keywordAuthor | Maltose binding protein-fused protein | - |
dc.subject.keywordAuthor | Human papillomavirus | - |
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