Litorimonas taeanensis gen. nov., sp nov., isolated from a sandy beach

Abstract

A heterotrophic, Gram-negative, prosthecate bacterium, designated strain G5(T), was isolated from a sandy beach of Taean in South Korea. Cells of strain G5(T) were aerobic, catalase- and oxidase-positive, straight to slightly curved motile rods with a single flagellum and formed yellow orange colonies on agar. Growth occurred at 15-40 degrees C (optimum 25-30 degrees C) and pH 6-9 (optimum pH 7-8). The major cellular fatty acids were C(18:1)omega 7c, C(17:0), C(16:0), 11-methyl C(18:1)omega 7c, C(17:1)omega 8c and C(17:1)omega 6c. The polar lipid pattern indicated the presence of phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids. The G+C content of the genomic DNA was 63.6 mol% and the major quinone was Q-10. Comparative 16S rRNA gene sequence analysis showed that strain G5(T) belonged to the branch containing the genera Hellea, Robiginitomaculum and Hypomonas within the family Hyphomonadaceae. Within this group, strain G5(T) was most closely related to Hellea balneolensis 26III/A02/215(T) with 95.8% 16S rRNA gene sequence similarity. Based on its phylogenetic position and its phenotypic, chemotaxonomic and molecular properties, strain G5(T) represents a novel species of a novel genus of the family Hyphomonadaceae, for which the name Litorimonas taeanensis gen. nov., sp. nov. is proposed. The type strain is G5(T) (=KACC 13701(T) =DSM 22008(T)).