IL-32 gamma Induces the Maturation of Dendritic Cells with Th1-and Th17-Polarizing Ability through Enhanced IL-12 and IL-6 Production
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Mi Young | - |
dc.contributor.author | Son, Mi Hye | - |
dc.contributor.author | Kim, Soo Hyun | - |
dc.contributor.author | Cho, Daeho | - |
dc.contributor.author | Kim, Tae Sung | - |
dc.date.accessioned | 2021-09-07T11:35:09Z | - |
dc.date.available | 2021-09-07T11:35:09Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-06-15 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112224 | - |
dc.description.abstract | IL-32, a newly described multifunctional cytokine, has been associated with a variety of inflammatory diseases, including rheumatoid arthritis, vasculitis, and Crohn's disease. In this study, we investigated the immunomodulatory effects of IL-32 gamma on bone marrow-derived dendritic cell (DC)-driven Th responses and analyzed the underlying signaling events. IL-32 gamma-treated DCs exhibited upregulated expression of cell-surface molecules and proinflammatory cytokines associated with DC maturation and activation. In particular, IL-32 gamma treatment significantly increased production of IL-12 and IL-6 in DCs, which are known as Th1- and Th17-polarizing cytokines, respectively. This increased production was inhibited by the addition of specific inhibitors of the activities of phospholipase C (PLC), JNK, and NF-kappa B. IL-32 gamma treatment increased the phosphorylation of JNK and the degradation of both I kappa B alpha and I kappa B beta in DCs, as well as NF-kappa B binding activity to the kappa B site. The PLC inhibitor suppressed NF-kappa B DNA binding activity and JNK phosphorylation increased by IL-32 gamma treatment, thereby indicating that IL-32 gamma induced IL-12 and IL-6 production in DCs via a PLC/JNK/NF-kappa B signaling pathway. Importantly, IL-32 gamma-stimulated DCs significantly induced both Th1 and Th17 responses when cocultured with CD4(+) T cells. The addition of a neutralizing anti-IL-12 mAb abolished the secretion of IFN-gamma in a dose-dependent manner; additionally, the blockage of IL-1 beta and IL-6, but not of IL-21 or IL-23p19, profoundly inhibited IL-32 gamma-induced IL-17 production. These results demonstrated that IL-32 gamma could effectively induce the maturation and activation of immature DCs, leading to enhanced Th1 and Th17 responses as the result of increased IL-12 and IL-6 production in DCs. The Journal of Immunology, 2011, 186: 6848-6859. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.subject | EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS | - |
dc.subject | IL-17-PRODUCING T-CELLS | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | IMMUNE-RESPONSES | - |
dc.subject | PROINFLAMMATORY CYTOKINE | - |
dc.subject | T(H)17 CELLS | - |
dc.subject | TH17 CELLS | - |
dc.subject | TGF-BETA | - |
dc.subject | INTERLEUKIN-12 | - |
dc.subject | DIFFERENTIATION | - |
dc.title | IL-32 gamma Induces the Maturation of Dendritic Cells with Th1-and Th17-Polarizing Ability through Enhanced IL-12 and IL-6 Production | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Sung | - |
dc.identifier.doi | 10.4049/jimmunol.1003996 | - |
dc.identifier.scopusid | 2-s2.0-79959560825 | - |
dc.identifier.wosid | 000291309700026 | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, v.186, no.12, pp.6848 - 6859 | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.citation.title | JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 186 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 6848 | - |
dc.citation.endPage | 6859 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS | - |
dc.subject.keywordPlus | IL-17-PRODUCING T-CELLS | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | PROINFLAMMATORY CYTOKINE | - |
dc.subject.keywordPlus | T(H)17 CELLS | - |
dc.subject.keywordPlus | TH17 CELLS | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | INTERLEUKIN-12 | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.