Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors
DC Field | Value | Language |
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dc.contributor.author | Li, Wenlin | - |
dc.contributor.author | Sun, Woong | - |
dc.contributor.author | Zhang, Yu | - |
dc.contributor.author | Wei, Wanguo | - |
dc.contributor.author | Ambasudhan, Rajesh | - |
dc.contributor.author | Xia, Peng | - |
dc.contributor.author | Talantova, Maria | - |
dc.contributor.author | Lin, Tongxiang | - |
dc.contributor.author | Kim, Janghwan | - |
dc.contributor.author | Wang, Xiaolei | - |
dc.contributor.author | Kim, Woon Ryoung | - |
dc.contributor.author | Lipton, Stuart A. | - |
dc.contributor.author | Zhang, Kang | - |
dc.contributor.author | Ding, Sheng | - |
dc.date.accessioned | 2021-09-07T12:22:14Z | - |
dc.date.available | 2021-09-07T12:22:14Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05-17 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112444 | - |
dc.description.abstract | Human embryonic stem cells (hESCs) hold enormous promise for regenerative medicine. Typically, hESC-based applications would require their in vitro differentiation into a desirable homogenous cell population. A major challenge of the current hESC differentiation paradigm is the inability to effectively capture and, in the long-term, stably expand primitive lineage-specific stem/precursor cells that retain broad differentiation potential and, more importantly, developmental stage-specific differentiation propensity. Here, we report synergistic inhibition of glycogen synthase kinase 3 (GSK3), transforming growth factor beta (TGF-beta), and Notch signaling pathways by small molecules can efficiently convert monolayer cultured hESCs into homogenous primitive neuroepithelium within 1 wk under chemically defined condition. These primitive neuroepithelia can stably self-renew in the presence of leukemia inhibitory factor, GSK3 inhibitor (CHIR99021), and TGF-beta receptor inhibitor (SB431542); retain high neurogenic potential and responsiveness to instructive neural patterning cues toward midbrain and hindbrain neuronal subtypes; and exhibit in vivo integration. Our work uniformly captures and maintains primitive neural stem cells from hESCs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | IN-VITRO | - |
dc.subject | HUMAN ES | - |
dc.subject | EPITHELIAL-CELLS | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | SPECIFICATION | - |
dc.subject | BRAIN | - |
dc.subject | STAGE | - |
dc.subject | MAINTENANCE | - |
dc.subject | ACTIVATION | - |
dc.subject | GENERATION | - |
dc.title | Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sun, Woong | - |
dc.contributor.affiliatedAuthor | Kim, Woon Ryoung | - |
dc.identifier.doi | 10.1073/pnas.1014041108 | - |
dc.identifier.scopusid | 2-s2.0-79957777958 | - |
dc.identifier.wosid | 000290719600047 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.108, no.20, pp.8299 - 8304 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 108 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 8299 | - |
dc.citation.endPage | 8304 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | HUMAN ES | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | SPECIFICATION | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | STAGE | - |
dc.subject.keywordPlus | MAINTENANCE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GENERATION | - |
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