HEAT SHOCK PROTEIN 27-TARGETED HEPTAPEPTIDE OF THE PKC Delta CATALYTIC V5 REGION SENSITIZES TUMORS WITH RADIO- AND CHEMORESISTANCE
DC Field | Value | Language |
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dc.contributor.author | Lee, Hae-June | - |
dc.contributor.author | Kim, Eun-Ho | - |
dc.contributor.author | Seo, Woo Duck | - |
dc.contributor.author | Choi, Tae Hyun | - |
dc.contributor.author | Cheon, Gi-Jeong | - |
dc.contributor.author | Lee, Yoon-Jin | - |
dc.contributor.author | Lee, Yun-Sil | - |
dc.date.accessioned | 2021-09-07T12:35:32Z | - |
dc.date.available | 2021-09-07T12:35:32Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05-01 | - |
dc.identifier.issn | 0360-3016 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112475 | - |
dc.description.abstract | Purpose: Previous data suggest that the PKC delta catalytic V5 (PKC delta-V5) heptapeptide (HEPT) (FEQFLDI) binds HSP27 and blocks HSP27-mediated radio- or chemoresistance. Here we investigated further the in vivo function of the PKC delta-V5 HEPT. Methods and Materials: Labeling of HEPT with Cy5.5 or fluorescein isothiocyanate was performed to evaluate in vitro or in vivo distribution of HEPT. A clonogenic survival assay, flow cytometry, and Western blotting of cleaved caspase-3 were performed to determine in vitro sensitization effects of HEPT plus ionizing radiation (IR) versus IR alone or those of HEPT plus cisplatin(Cis) versus Cis alone. A nude mouse xenografting system was also applied to detect in vivo sensitizing effects of HEPT. Results: HEPT efficiently bound to HSP27 and showed sensitization after combined treatment with IR versus treatment with Cis alone in NCI-H1299 lung carcinoma cells, with higher HSP27 expression, which was similar to that of combined treatment with IR or with Cis alone in NCI-H460 lung carcinoma cells with lower HSP27 expression. In vivo image analysis using Cy5.5-labeled HEPT showed that HEPT was retained in HSP27-overexpressing cancer cells after xenografting to nude mice. Combined treatment of HEPT with IR versus that with Cis alone in xenografted mice showed that HEPT increased radio- or chemosensitization in NCI-H1299 cells compared to that in mice xenografted with NCI-H460 cells. Conclusions: The novel PKC delta-V5 HEPT may help overcome HSP27-mediated radio- or chemoresistance. (C) 2011 Elsevier Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | RADIATION-INDUCED APOPTOSIS | - |
dc.subject | CELL-DEATH | - |
dc.subject | HSP27 | - |
dc.subject | INHIBITION | - |
dc.subject | RESISTANCE | - |
dc.subject | GROWTH | - |
dc.title | HEAT SHOCK PROTEIN 27-TARGETED HEPTAPEPTIDE OF THE PKC Delta CATALYTIC V5 REGION SENSITIZES TUMORS WITH RADIO- AND CHEMORESISTANCE | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cheon, Gi-Jeong | - |
dc.identifier.doi | 10.1016/j.ijrobp.2010.11.069 | - |
dc.identifier.scopusid | 2-s2.0-79954422840 | - |
dc.identifier.wosid | 000290006300032 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, v.80, no.1, pp.221 - 230 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS | - |
dc.citation.title | INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS | - |
dc.citation.volume | 80 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 221 | - |
dc.citation.endPage | 230 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.subject.keywordPlus | RADIATION-INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | HSP27 | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | Heptapeptide | - |
dc.subject.keywordAuthor | HSP27 | - |
dc.subject.keywordAuthor | PKC delta-V5 | - |
dc.subject.keywordAuthor | Tumor sensitization | - |
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