Lactoferrin inhibits the inflammatory and angiogenic activation of bovine aortic endothelial cells
- Authors
- Yeom, Mijung; Park, Jongbong; Lee, Bombi; Choi, Sang-Yun; Kim, Kyoung Soo; Lee, Hyejung; Hahm, Dae-Hyun
- Issue Date
- 5월-2011
- Publisher
- SPRINGER BASEL AG
- Keywords
- Lactoferrin; Endothelium; Bovine aortic endothelial cells (BAEC); Angiogenesis in vitro
- Citation
- INFLAMMATION RESEARCH, v.60, no.5, pp.475 - 482
- Indexed
- SCIE
SCOPUS
- Journal Title
- INFLAMMATION RESEARCH
- Volume
- 60
- Number
- 5
- Start Page
- 475
- End Page
- 482
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112485
- DOI
- 10.1007/s00011-010-0294-1
- ISSN
- 1023-3830
- Abstract
- Lactoferrin (Lf) is known to have anti-cancer and anti-inflammatory activities; however, its therapeutic mechanism has not been defined. In this study, to explain the therapeutic mechanism of Lf, we examined the effect of Lf on endothelial cell activation, leukocyte integration, and angiogenesis in vitro. Endothelia-leukocyte adhesion assays were used to assess primary cultures of bovine aortic endothelial cells (BAECs) activation following LPS treatment. The mRNA expression of ICAM-1 and proinflammatory cytokines was measured using RT-PCR. Each step of angiogenesis was evaluated in vitro, including endothelial cell proliferation, migration, and tube formation. Proliferation was examined using WST-1 and BrdU incorporation assays, while wound migration assays were used to evaluate cell migration; capillary-like tube formation assays on Matrigel were used to assess tube formation. Lf reduced the adhesion of human monocyte-like THP-1 cells to BAECs by 45%. Lf also reduced mRNA expression of ICAM-1 and proinflammatory cytokines in BAECs. Lf significantly inhibited BAEC proliferation, migration, and tube formation. Lf exerted a potent effect on BAEC activation, suggesting that it might function via an endothelia-based mechanism in the treatment of various diseases, including rheumatoid arthritis and cancer.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.