Apta-Biosensors for Nonlabeled Real Time Detection of Human IgE Based on Carbon Nanotube Field Effect Transistors
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jun Pyo | - |
dc.contributor.author | Hong, Seunghun | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2021-09-07T12:38:40Z | - |
dc.date.available | 2021-09-07T12:38:40Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112493 | - |
dc.description.abstract | In this study, we demonstrated the aptamer-based biosensor (apta-biosensor) using CNT-FET devices for label free detection of allergy diagnosis by IgE detection. In order to detect the IgE, two kinds of receptor (monoclonal IgE antibody and anti-IgE aptamer)-modified CNT-FET devices were fabricated. The binding event of the target IgE onto receptors was detected by monitoring the gating effect caused by the charges of the target proteins. Since the CNT-FET biosensors were used in buffer solution, it was crucial to use small-size receptors like aptamers than whole antibodies so that the charged target IgE could approach the CNT surface within the Debye length distance to give a large gating effect. The results show that CNT-FET biosensors using monoclonal IgE antibody had very low sensitivity (minimum detectable level similar to 1000 ng/mL), while those based on anti-IgE aptamer could detect 50 ng/mL. Moreover, the aptamer-modified CNT-FET herein could successfully block non-target proteins and could selectively detect the target protein in an environment similar to human serum electrolyte. Therefore, aptamer-based CNT-FET devices enable the production of label-free ultrasensitive electronic biosensors to detect clinically important biomarkers for disease diagnosis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.subject | SERUM IMMUNOGLOBULIN-E | - |
dc.subject | ANTIBODY | - |
dc.subject | FUNCTIONALIZATION | - |
dc.subject | NANOWIRES | - |
dc.subject | SELECTION | - |
dc.subject | DEVICES | - |
dc.title | Apta-Biosensors for Nonlabeled Real Time Detection of Human IgE Based on Carbon Nanotube Field Effect Transistors | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1166/jnn.2011.3692 | - |
dc.identifier.wosid | 000290692400063 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.11, no.5, pp.4182 - 4187 | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 11 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 4182 | - |
dc.citation.endPage | 4187 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | SERUM IMMUNOGLOBULIN-E | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | FUNCTIONALIZATION | - |
dc.subject.keywordPlus | NANOWIRES | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | DEVICES | - |
dc.subject.keywordAuthor | Carbon Nanotube | - |
dc.subject.keywordAuthor | Field Effect Transistor | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | IgE | - |
dc.subject.keywordAuthor | Allergy | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.