Multi-stage high cell continuous fermentation for high productivity and titer
DC Field | Value | Language |
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dc.contributor.author | Chang, Ho Nam | - |
dc.contributor.author | Kim, Nag-Jong | - |
dc.contributor.author | Kang, Jongwon | - |
dc.contributor.author | Jeong, Chang Moon | - |
dc.contributor.author | Choi, Jin-dal-rae | - |
dc.contributor.author | Fei, Qiang | - |
dc.contributor.author | Kim, Byoung Jin | - |
dc.contributor.author | Kwon, Sunhoon | - |
dc.contributor.author | Lee, Sang Yup | - |
dc.contributor.author | Kim, Jungbae | - |
dc.date.accessioned | 2021-09-07T12:48:34Z | - |
dc.date.available | 2021-09-07T12:48:34Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112547 | - |
dc.description.abstract | We carried out the first simulation on multi-stage continuous high cell density culture (MSC-HCDC) to show that the MSC-HCDC can achieve batch/fed-batch product titer with much higher productivity to the fed-batch productivity using published fermentation kinetics of lactic acid, penicillin and ethanol. The system under consideration consists of n-serially connected continuous stirred-tank reactors (CSTRs) with either hollow fiber cell recycling or cell immobilization for high cell-density culture. In each CSTR substrate supply and product removal are possible. Penicillin production is severely limited by glucose metabolite repression that requires multi-CSTR glucose feeding. An 8-stage C-HCDC lactic acid fermentation resulted in 212.9 g/L of titer and 10.6 g/L/h of productivity, corresponding to 101 and 429% of the comparable lactic acid fed-batch, respectively. The penicillin production model predicted 149% (0.085 g/L/h) of productivity in 8-stage C-HCDC with 40 g/L of cell density and 289% of productivity (0.165 g/L/h) in 7-stage C-HCDC with 60 g/L of cell density compared with referring batch cultivations. A 2-stage C-HCDC ethanol experimental run showed 107% titer and 257% productivity of the batch system having 88.8 g/L of titer and 3.7 g/L/h of productivity. MSC-HCDC can give much higher productivity than batch/fed-batch system, and yield a several percentage higher titer as well. The productivity ratio of MSC-HCDC over batch/fed-batch system is given as a multiplication of system dilution rate of MSC-HCDC and cycle time of batch/fed-batch system. We suggest MSC-HCDC as a new production platform for various fermentation products including monoclonal antibody. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | RECOMBINANT ANTIBODY-PRODUCTION | - |
dc.subject | ETHANOL FERMENTATIONS | - |
dc.subject | LACTIC-ACID | - |
dc.subject | DIRECTED EVOLUTION | - |
dc.subject | CULTURE | - |
dc.subject | DENSITY | - |
dc.subject | YEAST | - |
dc.subject | RECYCLE | - |
dc.subject | DESIGN | - |
dc.subject | PENICILLIN | - |
dc.title | Multi-stage high cell continuous fermentation for high productivity and titer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jungbae | - |
dc.identifier.doi | 10.1007/s00449-010-0485-8 | - |
dc.identifier.scopusid | 2-s2.0-79956208508 | - |
dc.identifier.wosid | 000289434700004 | - |
dc.identifier.bibliographicCitation | BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.34, no.4, pp.419 - 431 | - |
dc.relation.isPartOf | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.citation.title | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.citation.volume | 34 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 419 | - |
dc.citation.endPage | 431 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | RECOMBINANT ANTIBODY-PRODUCTION | - |
dc.subject.keywordPlus | ETHANOL FERMENTATIONS | - |
dc.subject.keywordPlus | LACTIC-ACID | - |
dc.subject.keywordPlus | DIRECTED EVOLUTION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | DENSITY | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | RECYCLE | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | PENICILLIN | - |
dc.subject.keywordAuthor | Multi-stage continuous fermentation | - |
dc.subject.keywordAuthor | High cell density | - |
dc.subject.keywordAuthor | Ethanol | - |
dc.subject.keywordAuthor | Lactic acid | - |
dc.subject.keywordAuthor | Antibody | - |
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