Purification and Characterization of an Extracellular beta-Glucosidase Produced by Phoma sp KCTC11825BP Isolated from Rotten Mandarin Peel
DC Field | Value | Language |
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dc.contributor.author | Choi, Jung-Youn | - |
dc.contributor.author | Park, Ah-Reum | - |
dc.contributor.author | Kim, Yong Jin | - |
dc.contributor.author | Kim, Jae-Jin | - |
dc.contributor.author | Cha, Chang-Jun | - |
dc.contributor.author | Yoon, Jeong-Jun | - |
dc.date.accessioned | 2021-09-07T12:50:08Z | - |
dc.date.available | 2021-09-07T12:50:08Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112555 | - |
dc.description.abstract | A beta-glucosidase from Phoma sp. KCTC11825BP isolated from rotten mandarin peel was purified 8.5-fold with a specific activity of 84.5 U/mg protein. The purified enzyme had a molecular mass of 440 kDa with a subunit of 110 kDa. The partial amino acid sequence of the purified beta-glucosidase evidenced high homology with the fungal beta-glucosidases belonging to glycosyl hydrolase family 3. Its optimal activity was detected at pH 4.5 and 60 degrees C, and the enzyme had a half-life of 53 h at 60 degrees C. The K-m for p-nitrophenyl-beta-D-glucopyranoside and cellobiose were 0.3 mM and 3.2 mM, respectively. The enzyme was competitively inhibited by both glucose (K-i=1.7 mM) and glucono-delta-lactone (K-i=0.1 mM) when pNPG was used as the substrate. Its activity was inhibited by 41% by 10 mM Cu2+ and stimulated by 20% by 10 mM Mg2+. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | MICROCRYSTALLINE CELLULOSE | - |
dc.subject | THERMOASCUS-AURANTIACUS | - |
dc.subject | HYDROLYSIS | - |
dc.subject | TOLERANT | - |
dc.subject | BINDING | - |
dc.title | Purification and Characterization of an Extracellular beta-Glucosidase Produced by Phoma sp KCTC11825BP Isolated from Rotten Mandarin Peel | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jae-Jin | - |
dc.identifier.doi | 10.4014/jmb.1102.02014 | - |
dc.identifier.scopusid | 2-s2.0-79958046959 | - |
dc.identifier.wosid | 000291130900008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.5, pp.503 - 508 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 21 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 503 | - |
dc.citation.endPage | 508 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001553018 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | MICROCRYSTALLINE CELLULOSE | - |
dc.subject.keywordPlus | THERMOASCUS-AURANTIACUS | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | TOLERANT | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | Phoma sp. | - |
dc.subject.keywordAuthor | beta-glucosidase | - |
dc.subject.keywordAuthor | identification | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | characterization | - |
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