Effects of microelectrical current on migration of nasal fibroblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hyuk | - |
dc.contributor.author | Cho, Jung-Sun | - |
dc.contributor.author | Park, Il Ho | - |
dc.contributor.author | Yoon, Hu Geun | - |
dc.contributor.author | Lee, Heung-Man | - |
dc.date.accessioned | 2021-09-07T12:51:30Z | - |
dc.date.available | 2021-09-07T12:51:30Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 1945-8924 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112563 | - |
dc.description.abstract | Background: Migration of fibroblasts is critical in wound healing. The question of how wounded electric fields guide migration of nasal fibroblasts remains to be elucidated. This study was designed to determine morphology, directedness, and migration rate of nasal fibroblasts during microcurrent application, which is simulated by an endogenous electric field at the vicinity of the wound. Methods: Nasal fibroblasts were exposed to a microelectric field at 50, 100, and 250 mV/mm for 3 hours at 37 degrees C. In this experiment, the field polarity was reversed for an additional 3 hours. During in vitro testing, the cells were incubated in a newly developed miniature, microcurrent generating chamber system, with 5% CO(2), at 37 degrees C; the media was circulated by a pump system. A wound was created by scratching a cell-free area (similar to 150 mu m wide) into a confluent monolayer. The average migration speed was calculated as the distance traveled by the cell divided by time. Results: A microelectric field of 100 mV/mm or more induced significant cell migration in the direction of the cathode. Trajectory speeds at 50, 100, and 250 mV/mm were 9.8 +/- 0.3, 11.8 +/- 0.3, and 13.5 +/- 0.9 mu m/mm, respectively. A significant difference was observed between migratory rate of controls and that of 50 mV/mm (p < 0.05). Conclusion: Microelectric fields appear to have a crucial role in control of nasal fibroblast activity in the process of wound healing. (Am J Rhinol Allergy 25, 157-162, 2011; doi: 10.2500/ajra.2011.25.3633) | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OCEAN SIDE PUBLICATIONS INC | - |
dc.subject | ENDOSCOPIC SINUS SURGERY | - |
dc.subject | CURRENT ELECTRIC-FIELDS | - |
dc.subject | EXTRACELLULAR-MATRIX MOLECULES | - |
dc.subject | CORNEAL EPITHELIAL-CELLS | - |
dc.subject | HUMAN DERMAL FIBROBLASTS | - |
dc.subject | HUMAN KERATINOCYTES | - |
dc.subject | WOUND MODEL | - |
dc.subject | IN-VITRO | - |
dc.subject | PROLIFERATION | - |
dc.subject | STIMULATION | - |
dc.title | Effects of microelectrical current on migration of nasal fibroblasts | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Hyuk | - |
dc.contributor.affiliatedAuthor | Park, Il Ho | - |
dc.contributor.affiliatedAuthor | Lee, Heung-Man | - |
dc.identifier.doi | 10.2500/ajra.2011.25.3633 | - |
dc.identifier.scopusid | 2-s2.0-79957496532 | - |
dc.identifier.wosid | 000292116500004 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, v.25, no.3, pp.157 - 162 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY | - |
dc.citation.title | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY | - |
dc.citation.volume | 25 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 157 | - |
dc.citation.endPage | 162 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.subject.keywordPlus | ENDOSCOPIC SINUS SURGERY | - |
dc.subject.keywordPlus | CURRENT ELECTRIC-FIELDS | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX MOLECULES | - |
dc.subject.keywordPlus | CORNEAL EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | HUMAN DERMAL FIBROBLASTS | - |
dc.subject.keywordPlus | HUMAN KERATINOCYTES | - |
dc.subject.keywordPlus | WOUND MODEL | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | STIMULATION | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.