Ataxin-1 occupies the promoter region of E-cadherin in vivo and activates CtBP2-repressed promoter
DC Field | Value | Language |
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dc.contributor.author | Lee, Soyeon | - |
dc.contributor.author | Hong, Sunghoi | - |
dc.contributor.author | Kim, Sungsu | - |
dc.contributor.author | Kang, Seongman | - |
dc.date.accessioned | 2021-09-07T12:52:12Z | - |
dc.date.available | 2021-09-07T12:52:12Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 0167-4889 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112567 | - |
dc.description.abstract | Ataxin-1 is a polyglutamine protein of unknown function that is encoded by the ATXN1 gene in humans. To gain insight into the function of ataxin-1, we sought to identify proteins that interact with ataxin-1 through yeast two-hybrid screening. In this study, transcriptional corepressor CtBP2 was identified as a protein that interacted with ataxin-1. CtBP2 and ataxin-1 colocalized in the nucleus of mammalian cells. Since the E-cadherin promoter is a target of CtBP-mediated repression, the relationship between ataxin-1 and the E-cadherin promoter was investigated. Chromatin immunoprecipitation assays showed that CtBP2 and ataxin-1 were recruited to the E-cadherin promoter in mammalian cells. Luciferase assays using E-cadherin promoter reporter constructs revealed that the luciferase activity was enhanced as the level of ataxin-1 protein expression increased. CtBP2 overexpression decreased E-cadherin expression, but expression of ataxin-1 inversely increased the mRNA and protein levels of endogenous E-cadherin. Interestingly, siRNA experiments showed that the transcriptional activation of ataxin-1 was associated with the presence of CtBP2. This study demonstrates that ataxin-1 occupies the promoter region of E-cadherin in vivo and that ataxin-1 activates the promoter in a CtBP2-mediated transcriptional regulation manner. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | GENE-PRODUCT ATAXIN-1 | - |
dc.subject | BINDING-PROTEIN | - |
dc.subject | TRANSCRIPTIONAL REPRESSION | - |
dc.subject | COREPRESSOR CTBP | - |
dc.subject | CO-REPRESSOR | - |
dc.subject | TUMOR-CELLS | - |
dc.subject | EXPRESSION | - |
dc.subject | INTERACTS | - |
dc.subject | SCA1 | - |
dc.subject | SNAIL | - |
dc.title | Ataxin-1 occupies the promoter region of E-cadherin in vivo and activates CtBP2-repressed promoter | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Sunghoi | - |
dc.contributor.affiliatedAuthor | Kang, Seongman | - |
dc.identifier.doi | 10.1016/j.bbamcr.2011.01.035 | - |
dc.identifier.scopusid | 2-s2.0-79955661923 | - |
dc.identifier.wosid | 000291125300006 | - |
dc.identifier.bibliographicCitation | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v.1813, no.5, pp.713 - 722 | - |
dc.relation.isPartOf | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | - |
dc.citation.title | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | - |
dc.citation.volume | 1813 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 713 | - |
dc.citation.endPage | 722 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | GENE-PRODUCT ATAXIN-1 | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL REPRESSION | - |
dc.subject.keywordPlus | COREPRESSOR CTBP | - |
dc.subject.keywordPlus | CO-REPRESSOR | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INTERACTS | - |
dc.subject.keywordPlus | SCA1 | - |
dc.subject.keywordPlus | SNAIL | - |
dc.subject.keywordAuthor | Ataxin-1 | - |
dc.subject.keywordAuthor | CtBP2 | - |
dc.subject.keywordAuthor | E-cadherin | - |
dc.subject.keywordAuthor | Repression | - |
dc.subject.keywordAuthor | Activation | - |
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