A novel alkaline lipase from Ralstonia with potential application in biodiesel production
DC Field | Value | Language |
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dc.contributor.author | Yoo, Hah-Young | - |
dc.contributor.author | Simkhada, Jaya Ram | - |
dc.contributor.author | Cho, Seung Sik | - |
dc.contributor.author | Park, Don Hee | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.contributor.author | Seong, Chi Nam | - |
dc.contributor.author | Yoo, Jin Cheol | - |
dc.date.accessioned | 2021-09-07T13:04:34Z | - |
dc.date.available | 2021-09-07T13:04:34Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-05 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112614 | - |
dc.description.abstract | With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000 Da by SDS-PAGE. The activity was highest at 50-55 degrees C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45 degrees C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and K-m and V-max for p-nitrophenyl palmitate were 2.73 +/- 0.6 mM and 101.4 +/- 1.9 mM/min mg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | EXTRACELLULAR LIPASE | - |
dc.subject | PURIFICATION | - |
dc.subject | TRANSESTERIFICATION | - |
dc.subject | METHANOL | - |
dc.title | A novel alkaline lipase from Ralstonia with potential application in biodiesel production | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.identifier.doi | 10.1016/j.biortech.2011.02.046 | - |
dc.identifier.scopusid | 2-s2.0-79955001309 | - |
dc.identifier.wosid | 000291125800075 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.102, no.10, pp.6104 - 6111 | - |
dc.relation.isPartOf | BIORESOURCE TECHNOLOGY | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 102 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 6104 | - |
dc.citation.endPage | 6111 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | EXTRACELLULAR LIPASE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | TRANSESTERIFICATION | - |
dc.subject.keywordPlus | METHANOL | - |
dc.subject.keywordAuthor | Ralstonia sp CS274 | - |
dc.subject.keywordAuthor | Lipase | - |
dc.subject.keywordAuthor | Purification | - |
dc.subject.keywordAuthor | Biodiesel production | - |
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