Solution structure of the Z beta domain of human DNA-dependent activator of IFN-regulatory factors and its binding modes to B- and Z-DNAs
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyungmin | - |
dc.contributor.author | Khayrutdinov, Bulat I. | - |
dc.contributor.author | Lee, Chung-Kyung | - |
dc.contributor.author | Cheong, Hae-Kap | - |
dc.contributor.author | Kang, Sung Wook | - |
dc.contributor.author | Park, Hyejin | - |
dc.contributor.author | Lee, Sangho | - |
dc.contributor.author | Kim, Yang-Gyun | - |
dc.contributor.author | Jee, JunGoo | - |
dc.contributor.author | Rich, Alexander | - |
dc.contributor.author | Kim, Kyeong Kyu | - |
dc.contributor.author | Jeon, Young Ho | - |
dc.date.accessioned | 2021-09-07T13:14:19Z | - |
dc.date.available | 2021-09-07T13:14:19Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-04-26 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112632 | - |
dc.description.abstract | The DNA-dependent activator of IFN-regulatory factors (DAI), also known as DLM-1/ZBP1, initiates an innate immune response by binding to foreign DNAs in the cytosol. For full activation of the immune response, three DNA binding domains at the N terminus are required: two Z-DNA binding domains (ZBDs), Z alpha and Z beta, and an adjacent putative B-DNA binding domain. The crystal structure of the Z beta domain of human DAI (hZ beta(DAI)) in complex with Z-DNA revealed structural features distinct from other known Z-DNA binding proteins, and it was classified as a group II ZBD. To gain structural insights into the DNA binding mechanism of hZ beta(DAI), the solution structure of the free hZ beta(DAI) was solved, and its bindings to B-and Z-DNAs were analyzed by NMR spectroscopy. Compared to the Z-DNA-bound structure, the conformation of free hZ beta(DAI) has notable alterations in the alpha 3 recognition helix, the "wing," and Y145, which are critical in Z-DNA recognition. Unlike some other Za domains, hZ beta(DAI) appears to have conformational flexibility, and structural adaptation is required for Z-DNA binding. Chemical-shift perturbation experiments revealed that hZ beta(DAI) also binds weakly to B-DNA via a different binding mode. The C-terminal domain of DAI is reported to undergo a conformational change on B-DNA binding; thus, it is possible that these changes are correlated. During the innate immune response, hZ beta(DAI) is likely to play an active role in binding to DNAs in both B and Z conformations in the recognition of foreign DNAs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | Z-ALPHA DOMAIN | - |
dc.subject | HANDED Z-DNA | - |
dc.subject | INNATE IMMUNE-RESPONSE | - |
dc.subject | ENZYME ADAR1 REVEALS | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | DIPOLAR COUPLINGS | - |
dc.subject | DAI DLM-1/ZBP1 | - |
dc.subject | PROTEINS | - |
dc.subject | COMPLEX | - |
dc.subject | FAMILY | - |
dc.title | Solution structure of the Z beta domain of human DNA-dependent activator of IFN-regulatory factors and its binding modes to B- and Z-DNAs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeon, Young Ho | - |
dc.identifier.doi | 10.1073/pnas.1014898107 | - |
dc.identifier.scopusid | 2-s2.0-79955567592 | - |
dc.identifier.wosid | 000289888500048 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.108, no.17, pp.6921 - 6926 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 108 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 6921 | - |
dc.citation.endPage | 6926 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | Z-ALPHA DOMAIN | - |
dc.subject.keywordPlus | HANDED Z-DNA | - |
dc.subject.keywordPlus | INNATE IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | ENZYME ADAR1 REVEALS | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | DIPOLAR COUPLINGS | - |
dc.subject.keywordPlus | DAI DLM-1/ZBP1 | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | FAMILY | - |
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