Overexpression of IL-32 alpha Increases Natural Killer Cell-mediated Killing through Up-regulation of Fas and UL16-binding protein 2 (ULBP2) Expression in Human Chronic Myeloid Leukemia Cells
DC Field | Value | Language |
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dc.contributor.author | Cheon, Soyoung | - |
dc.contributor.author | Lee, Ji Hyung | - |
dc.contributor.author | Park, Sunyoung | - |
dc.contributor.author | Bang, Sa Ik | - |
dc.contributor.author | Lee, Wang Jae | - |
dc.contributor.author | Yoon, Do-Young | - |
dc.contributor.author | Yoon, Sung-Soo | - |
dc.contributor.author | Kim, Taesung | - |
dc.contributor.author | Min, Hyeyoung | - |
dc.contributor.author | Cho, Byung Joo | - |
dc.contributor.author | Lee, Hyong Joo | - |
dc.contributor.author | Lee, Ki Woong | - |
dc.contributor.author | Jeong, Seung Hwan | - |
dc.contributor.author | Park, Hyunjeong | - |
dc.contributor.author | Cho, Daeho | - |
dc.date.accessioned | 2021-09-07T13:21:21Z | - |
dc.date.available | 2021-09-07T13:21:21Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-04-08 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112672 | - |
dc.description.abstract | IL-32 was recently identified as a proinflammatory cytokine that is induced by IL-18 in natural killer (NK) cells and is highly correlated with inflammatory disorders. However, the relationship between IL-32 and tumor progression is still unknown. In this study, we investigated whether overexpression of IL-32 affects susceptibility of chronic myeloid leukemia (CML) cells to NK cells. Interestingly, IL-32 alpha-overexpressing CML cell lines, K562, Kcl22, and BV173, showed higher NK cell-mediated killing. Flow cytometry analysis revealed that overexpression of IL-32 alpha induced increased expression of Fas and UL16-binding protein 2 (ULBP2) in CML cells. The direct relationship between overexpression of surface molecules by IL-32 alpha and increased NK cell-mediated killing was confirmed by Fas or ULBP2 siRNA transfection. IL-32 alpha-induced Fas and ULBP2 expression are regulated p38 MAPK. In addition, the transcription factor Ets1 plays a key role in ULBP2 specific expression by IL-32 alpha overexpression in ULBP family members. Taken together, these data show that IL-32 alpha stimulates Fas and ULBP2 expression via activation of p38 MAPK, which increases NK susceptibility of CML cells. Enhanced NK cell susceptibility of CML cells by IL-32 alpha overexpression may improve the efficiency of NK cell-based immunotherapy. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | NKG2D IMMUNORECEPTOR | - |
dc.subject | T-CELLS | - |
dc.subject | INTERLEUKIN-32 | - |
dc.subject | ACTIVATION | - |
dc.subject | CANCER | - |
dc.subject | LIGANDS | - |
dc.subject | ROLES | - |
dc.subject | IL-32 | - |
dc.subject | APOPTOSIS | - |
dc.subject | CYTOKINE | - |
dc.title | Overexpression of IL-32 alpha Increases Natural Killer Cell-mediated Killing through Up-regulation of Fas and UL16-binding protein 2 (ULBP2) Expression in Human Chronic Myeloid Leukemia Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Taesung | - |
dc.identifier.doi | 10.1074/jbc.M110.159756 | - |
dc.identifier.scopusid | 2-s2.0-79953301760 | - |
dc.identifier.wosid | 000289077500019 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.286, no.14 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 286 | - |
dc.citation.number | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | NKG2D IMMUNORECEPTOR | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | INTERLEUKIN-32 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | LIGANDS | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | IL-32 | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CYTOKINE | - |
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