A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase E has synergistic effects on galactomannan degradation
- Authors
- Jeon, Sang Duck; Yu, Kyung Ok; Kim, Seung Wook; Han, Sung Ok
- Issue Date
- 4월-2011
- Publisher
- SPRINGER
- Keywords
- Mannanase; Cellulosome; Clostridium cellulovorans; Synergistic effect; Galactomannan
- Citation
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.90, no.2, pp.565 - 572
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 90
- Number
- 2
- Start Page
- 565
- End Page
- 572
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112746
- DOI
- 10.1007/s00253-011-3108-7
- ISSN
- 0175-7598
- Abstract
- In our previous study using a fluorescently labeled cohesin biomarker, we detected and identified a putative cellulosomal mannanase belonging to the glycosyl hydrolase family 26 from Clostridium cellulovorans in xylan-containing cultures. In this study, a mannanase gene, manB from C. cellulovorans, was expressed in Escherichia coli. The optimal pH of a purified enzyme was around pH 7.0 and the optimal temperature was 40A degrees C. The purified mannanase B (ManB) showed high hydrolytic activity toward galactomannan. An assembly of ManB with mini-CbpA, which contains a carbohydrate-binding module that provides proximity to insoluble substrates, increased the activity toward galactomannan [locust bean gum (LBG) and guar gum] 1.7- and 2.0-fold over those without mini-CbpA. We tested the synergistic effects on galactomannan (LBG and guar gum) degradation using cellulosomal mannanase ManB with cellulosomal endoglucanase E, which was predicted to have mannanase activity in C. cellulovorans as a cellulolytic complex. When assembled with the mini-CbpA, the mixture of endoglucanase E (EngE) and ManB at a molar ratio of 1:2 showed the highest synergistic effect (2.4-fold) on LBG. The mixture at a ratio of 1:3 showed the highest synergistic effect (2.8-fold) on guar gum. These synergistic actions indicated that ManB assembled with mini-CbpA hydrolyzed insoluble galactomannan, which in turn promoted soluble galactomannan degradation by EngE.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
- Graduate School > Department of Biotechnology > 1. Journal Articles
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