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A Simple and Reliable Method for Obtaining Entomosporium Monoconidial Isolates

Authors
Park, Mi-JeongHoltslag, Quinn A.Shin, Hyeon-Dong
Issue Date
4월-2011
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Keywords
Entomosporium mespili; fan-shaped colony; germination; isolation technique
Citation
JOURNAL OF MICROBIOLOGY, v.49, no.2, pp.324 - 326
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF MICROBIOLOGY
Volume
49
Number
2
Start Page
324
End Page
326
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/112800
DOI
10.1007/s12275-011-1117-y
ISSN
1225-8873
Abstract
Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and transferring the characteristic fan-shaped colonies to fresh medium.
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College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles

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