Salmonella typhimurium harboring plasmid expressing interleukin-12 induced attenuation of infection and protective immune responses
DC Field | Value | Language |
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dc.contributor.author | Yoon, Won Suck | - |
dc.contributor.author | Choi, Hyuk Jin | - |
dc.contributor.author | Park, Yong Keun | - |
dc.date.accessioned | 2021-09-07T13:49:23Z | - |
dc.date.available | 2021-09-07T13:49:23Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-04 | - |
dc.identifier.issn | 0022-1260 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112803 | - |
dc.description.abstract | IL-12 is known to be an essential cytokine which appears to provide protective immunity against intracellular bacteria, such as Salmonella. In this study, we investigated the possibility of developing a vaccine using IL-12 against virulent Salmonella. We used the host defense system activated by cytokine IL-12. The highly virulent Salmonella strain (Salmonella typhimurium UK-1) was transformed with cytokine-expressing plasmids. These live, wild-type pathogens were used as vaccine strains without undergoing any other biological or genetic attenuating processes. The newly developed strains induced partial protection from infections (30-40%). Of note, the interleukin-12-transformed pathogen was safe upon immunization with low doses (10(3) cfu), induced IgG responses, and stimulated protective immune responses against Salmonella typhimurium in mice (80-100%). These results suggest that IL-12 induced attenuation of wild-type Salmonella in the host infection stage and vaccine development using the wild-type strain harboring plasmid-secreting IL-12 may be considered as an alternative process for intracellular bacterial vaccine development without the inconvenience of time-consuming attenuation processes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MICROBIOL RES FOUNDATION | - |
dc.subject | T-CELL RESPONSES | - |
dc.subject | IFN-GAMMA | - |
dc.subject | VACCINES | - |
dc.subject | PROTEIN | - |
dc.subject | IL-12 | - |
dc.title | Salmonella typhimurium harboring plasmid expressing interleukin-12 induced attenuation of infection and protective immune responses | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoon, Won Suck | - |
dc.contributor.affiliatedAuthor | Park, Yong Keun | - |
dc.identifier.doi | 10.2323/jgam.57.115 | - |
dc.identifier.scopusid | 2-s2.0-79957869701 | - |
dc.identifier.wosid | 000296949100005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, v.57, no.2, pp.115 - 122 | - |
dc.relation.isPartOf | JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | - |
dc.citation.title | JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | - |
dc.citation.volume | 57 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 115 | - |
dc.citation.endPage | 122 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | T-CELL RESPONSES | - |
dc.subject.keywordPlus | IFN-GAMMA | - |
dc.subject.keywordPlus | VACCINES | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | IL-12 | - |
dc.subject.keywordAuthor | attenuation | - |
dc.subject.keywordAuthor | interleukin-12 | - |
dc.subject.keywordAuthor | Salmonella typhimurium | - |
dc.subject.keywordAuthor | vaccine | - |
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