CD8(+) T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyo-Jin | - |
dc.contributor.author | Jung, Bong-Kwang | - |
dc.contributor.author | Lee, Jin-Joo | - |
dc.contributor.author | Pyo, Kyoung-Ho | - |
dc.contributor.author | Kim, Tae Yun | - |
dc.contributor.author | Choi, Byung-il | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Hisaeda, Hajime | - |
dc.contributor.author | Himeno, Kunisuke | - |
dc.contributor.author | Shin, Eun-Hee | - |
dc.contributor.author | Chai, Jong-Yil | - |
dc.date.accessioned | 2021-09-07T14:23:27Z | - |
dc.date.available | 2021-09-07T14:23:27Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/112883 | - |
dc.description.abstract | Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8(+) T-cells and CD4(+) T-cells. However, in mice immunized using a gene gun, significantly higher (P < 0.05) proportions of CD8(+) cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI | - |
dc.subject | APICAL MEMBRANE ANTIGEN-1 | - |
dc.subject | CANDIDATE | - |
dc.subject | GENE | - |
dc.subject | IMMUNOGENICITY | - |
dc.subject | EXPRESSION | - |
dc.subject | PROTEIN | - |
dc.subject | DOMAIN | - |
dc.title | CD8(+) T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Woo | - |
dc.identifier.doi | 10.3347/kjp.2011.49.1.85 | - |
dc.identifier.scopusid | 2-s2.0-79953285353 | - |
dc.identifier.wosid | 000289540600014 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PARASITOLOGY, v.49, no.1, pp.85 - 90 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.citation.title | KOREAN JOURNAL OF PARASITOLOGY | - |
dc.citation.volume | 49 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 85 | - |
dc.citation.endPage | 90 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Parasitology | - |
dc.relation.journalWebOfScienceCategory | Parasitology | - |
dc.subject.keywordPlus | APICAL MEMBRANE ANTIGEN-1 | - |
dc.subject.keywordPlus | CANDIDATE | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | IMMUNOGENICITY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordAuthor | Plasmodium vivax | - |
dc.subject.keywordAuthor | DNA vaccine | - |
dc.subject.keywordAuthor | apical membrane antigen (AMA) | - |
dc.subject.keywordAuthor | CD8(+) T-cell | - |
dc.subject.keywordAuthor | gene gun | - |
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