Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

A genetic screen for leaf movement mutants identifies a potential role for AGAMOUS-LIKE 6 (AGL6) in circadian-clock control

Authors
Yoo, Seung KwanHong, Sung MyunLee, Jong SeobAhn, Ji Hoon
Issue Date
3월-2011
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Keywords
AGL6; CCA1; circadian clock; leaf movement; ZTL
Citation
MOLECULES AND CELLS, v.31, no.3, pp.281 - 287
Indexed
SCIE
SCOPUS
KCI
Journal Title
MOLECULES AND CELLS
Volume
31
Number
3
Start Page
281
End Page
287
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/113004
DOI
10.1007/s10059-011-0035-5
ISSN
1016-8478
Abstract
The circadian clock in plants regulates many important physiological and biological processes, including leaf movement. We have used an imaging system to genetically screen Arabidopsis seedlings for altered leaf movement with the aim of identifying a circadian clock gene. A total of 285 genes were selected from publicly available microarrays that showed an expression pattern similar to those of the Arabidopsis core oscillator genes. We subsequently isolated 42 homozygous recessive mutants and analyzed their leaf movements. We also analyzed leaf movements of activation tagging mutants that showed altered flowering time. We found that agl6-1D plants, in which AGAMOUS-LIKE 6 (AGL6) was activated by the 35S enhancer, showed a shortened period of leaf movement as well as a high level of ZEITLUPE (ZTL) expression, reduced amplitude of LATE ELONGATED HYPOCOTYL (LHY) expression, and arrhythmic TIMING OF CAB EXPRESSION1 (TOC1)/CIRCADIAN CLOCK ASSOCIATED1 (CCA1) expression. A shortened period of leaf movement was also seen in 35S-AGL6-myc plants, although 35S-amiRAGL6 plants, transgenic plants overexpressing an artificial miRNA (amiR) targeting AGL6, showed unaltered leaf movement. The amplitude of CHLOROPHYLL A/B BINDING PROTEIN 2 (CAB2) expression, a circadian output gene, was also reduced in agl6-1D plants. Taken together, these results suggest that AGL6 plays a potential role in the regulation of the circadian clock by regulating ZTL mRNA level in Arabidopsis.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Life Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Ahn, Ji Hoon photo

Ahn, Ji Hoon
분자생명과학과
Read more

Altmetrics

Total Views & Downloads

BROWSE