Enhancement of DNA vaccine potency by antigen linkage to IFN-gamma-inducible protein-10
- Authors
- Kang, Tae Heung; Kim, Keon Woo; Bae, Hyun Cheol; Seong, Seung-Yong; Kim, Tae Woo
- Issue Date
- 1-2월-2011
- Publisher
- WILEY
- Keywords
- DNA vaccines; chemokines; IP-10; HPV-16 E7; CD4(+) Th1 T-cells; CD8(+) T-cells
- Citation
- INTERNATIONAL JOURNAL OF CANCER, v.128, no.3, pp.702 - 714
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF CANCER
- Volume
- 128
- Number
- 3
- Start Page
- 702
- End Page
- 714
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113093
- DOI
- 10.1002/ijc.25391
- ISSN
- 0020-7136
- Abstract
- DNA vaccines have emerged as an attractive approach to generate antigen-specific T-cell immune response. Nevertheless, the potency of DNA vaccines still needs to be improved for cancer immunotherapy. In this study, we explored whether functional linkage of a Th1-polarizing chemokine, IP-10, to a model tumor antigen, human papillomavirus type 16 (HPV-16) E7, enhanced DNA vaccine potency. IP-10 linkage changed the location of E7 from the nucleus to the endoplasmic reticulum and led to the secretion of functionally chemoattractive chimeric IP-10/E7 protein. In addition, this linkage drastically enhanced the endogenous processing of E7 antigen through MHC class I. More importantly, we found that C57BL/6 mice intradermally vaccinated with IP-10/E7 DNA exhibited a dramatic increase in the number of E7-specific CD4(+) Th1 T-cells and CD8(+) T-cells and, consequently, were strongly resistant over the long term to E7-expressing tumors compared to mice vaccinated with wild-type E7 DNA. Thus, because of the increase in tumor antigen-specific T-cell immune responses obtained through both enhanced antigen presentation and chemoattraction, vaccination with DNA encoding IP-10 linked to a tumor antigen holds great promise for treating tumors.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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