In Vivo Evaluation of Mixtures of Uncultured Freshly Isolated Adipose-Derived Stem Cells and Demineralized Bone Matrix for Bone Regeneration in a Rat Critically Sized Calvarial Defect Model
DC Field | Value | Language |
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dc.contributor.author | Rhee, Seung Chul | - |
dc.contributor.author | Ji, Yi-hwa | - |
dc.contributor.author | Gharibjanian, Nareg A. | - |
dc.contributor.author | Dhong, Eun Sang | - |
dc.contributor.author | Park, Seung Ha | - |
dc.contributor.author | Yoon, Eul-Sik | - |
dc.date.accessioned | 2021-09-07T15:49:44Z | - |
dc.date.available | 2021-09-07T15:49:44Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-02 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/113194 | - |
dc.description.abstract | Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX (R)) and/or poly(70L-lactide-co-30DL-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n = 10): (1) control, (2) DBM, (3) DBM + SVF, (4) DBM + PLA, and (5) DBM + PLA + SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM + SVF and DBM + PLA + SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P = 0.030 < 0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM + SVF: 57.69%; DBM + PLA: 24.86%; DBM + PLA + SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM + SVF and DBM + PLA + SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.subject | POLY-L/DL-LACTIDE | - |
dc.subject | STROMAL CELLS | - |
dc.subject | SPINE FUSION | - |
dc.subject | TISSUE | - |
dc.subject | MARROW | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | CAPACITY | - |
dc.subject | IMPLANTS | - |
dc.subject | FATTY | - |
dc.title | In Vivo Evaluation of Mixtures of Uncultured Freshly Isolated Adipose-Derived Stem Cells and Demineralized Bone Matrix for Bone Regeneration in a Rat Critically Sized Calvarial Defect Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Dhong, Eun Sang | - |
dc.contributor.affiliatedAuthor | Park, Seung Ha | - |
dc.contributor.affiliatedAuthor | Yoon, Eul-Sik | - |
dc.identifier.doi | 10.1089/scd.2009.0525 | - |
dc.identifier.scopusid | 2-s2.0-78951477715 | - |
dc.identifier.wosid | 000286460000006 | - |
dc.identifier.bibliographicCitation | STEM CELLS AND DEVELOPMENT, v.20, no.2, pp.233 - 242 | - |
dc.relation.isPartOf | STEM CELLS AND DEVELOPMENT | - |
dc.citation.title | STEM CELLS AND DEVELOPMENT | - |
dc.citation.volume | 20 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 233 | - |
dc.citation.endPage | 242 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | POLY-L/DL-LACTIDE | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | SPINE FUSION | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | MARROW | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | CAPACITY | - |
dc.subject.keywordPlus | IMPLANTS | - |
dc.subject.keywordPlus | FATTY | - |
dc.subject.keywordAuthor | stem cell | - |
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