Influence of Different Substrates in Wetland Soils on Denitrification

Abstract

Different substrates were evaluated to investigate their effect on nitrate removal and denitrifying bacterial community in soils obtained from wetland. Serial batch kinetic tests were conducted on soils obtained from wetland mixed with glucose and sawdust using KNO3 solution. Column tests were also conducted on soils obtained from wetland mixed with three different substrates (glucose, sawdust, and scoria coated with zero-valent iron) using KNO3 solution. For the batch tests, the nitrate removal efficiency for soil mixed with glucose was comparable to that for soil mixed with sawdust, but the nitrate removal rate for soil mixed with glucose (23.3 NO (3) (-) -N mg/L-d) was approximately eight times higher than that for soil mixed with sawdust (2.8 NO (3) (-) -N mg/L-d). For column tests among soil samples, nitrate removal efficiency was highest in soil mixed with glucose, which is an easily biodegradable carbon source. Removal efficiency increased with increasing incubation time for both soil samples with glucose and sawdust. A phylogenetic analysis based on nitrate reductase gene demonstrated that the different carbon sources affected both the diversity and compositions of the denitrifying bacterial in soil samples.