Acinetobacter oleivorans sp nov Is Capable of Adhering to and Growing on Diesel-Oil

Abstract

A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Grain-negative, aerobic coccobacilli, and grew at 20-37 degrees C with the optimal temperature of 30 degrees C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 4:1.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a,novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)).