Cancer stem cell traits in squamospheres derived from primary head and neck squamous cell carcinomas
DC Field | Value | Language |
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dc.contributor.author | Lim, Young Chang | - |
dc.contributor.author | Oh, Se-Yeong | - |
dc.contributor.author | Cha, Yun Yi | - |
dc.contributor.author | Kim, Sung-Hak | - |
dc.contributor.author | Jin, Xun | - |
dc.contributor.author | Kim, Hyunggee | - |
dc.date.accessioned | 2021-09-07T15:56:01Z | - |
dc.date.available | 2021-09-07T15:56:01Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-02 | - |
dc.identifier.issn | 1368-8375 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/113230 | - |
dc.description.abstract | A subpopulation of cancer stem cells (CSCs), but not the majority of non-tumorigenic cancer cells, in a variety of human malignancies plays a critical role in cancer cell proliferation, invasion, metastasis, and tumor recurrence post-therapies. We report the isolation of sphere-forming cells (squamospheres) from primary head and neck squamous cell carcinomas (HNSCCs), and characterization of their CSC properties. Squamospheres appeared within 2 weeks after seeding as single-dissociated cells obtained from primary HNSCC specimens in serum-free culture conditions. Real-time RT-PCR and immunocytochemistry assays revealed that a number of stem cell markers, including CK5, OCT4, SOX2, and nestin, were up-regulated in HNSCC-driven squamospheres. Fluorescence-activated cell sorting (FACS) analysis showed that squamospheres contain enriched side population cells compared to serum-induced differentiated squamosphere cells. Furthermore, HNSCC-driven squamospheres appeared to be chemoresistant to cisplatin, 5-fluorouracil (FU), paclitaxel and doxetaxel, and showed increased levels of ABCG2, one of the ATP-binding cassette (ABC) transporters. Of particular interest, in sharp contrast to subcutaneous injection of 1 x 10(6) differentiated squamosphere cells, as few as 100 squamosphere cells were able to give rise to tumors in nude mice. Altogether, we assert that primary HNSCC-driven squamospheres possess CSC properties, and its functional analysis may provide a novel tool for investigating the tumorigenic process of HNSCC. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | PROSPECTIVE IDENTIFICATION | - |
dc.subject | DRUG-RESISTANCE | - |
dc.subject | LUNG-CANCER | - |
dc.subject | TUMORS | - |
dc.subject | CD133 | - |
dc.subject | LINE | - |
dc.title | Cancer stem cell traits in squamospheres derived from primary head and neck squamous cell carcinomas | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Sung-Hak | - |
dc.contributor.affiliatedAuthor | Jin, Xun | - |
dc.contributor.affiliatedAuthor | Kim, Hyunggee | - |
dc.identifier.doi | 10.1016/j.oraloncology.2010.11.011 | - |
dc.identifier.scopusid | 2-s2.0-79551686424 | - |
dc.identifier.wosid | 000286928800001 | - |
dc.identifier.bibliographicCitation | ORAL ONCOLOGY, v.47, no.2, pp.83 - 91 | - |
dc.relation.isPartOf | ORAL ONCOLOGY | - |
dc.citation.title | ORAL ONCOLOGY | - |
dc.citation.volume | 47 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 83 | - |
dc.citation.endPage | 91 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Dentistry, Oral Surgery & Medicine | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Dentistry, Oral Surgery & Medicine | - |
dc.subject.keywordPlus | PROSPECTIVE IDENTIFICATION | - |
dc.subject.keywordPlus | DRUG-RESISTANCE | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | TUMORS | - |
dc.subject.keywordPlus | CD133 | - |
dc.subject.keywordPlus | LINE | - |
dc.subject.keywordAuthor | Neoplastic stem cells | - |
dc.subject.keywordAuthor | Head and neck neoplasms | - |
dc.subject.keywordAuthor | Squamospheres | - |
dc.subject.keywordAuthor | Oral cancer | - |
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