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High-speed synthetic aperture microscopy for live cell imaging

Authors
Kim, MoonseokChoi, YoungwoonFang-Yen, ChristopherSung, YongjinDasari, Ramachandra R.Feld, Michael S.Choi, Wonshik
Issue Date
15-1월-2011
Publisher
OPTICAL SOC AMER
Citation
OPTICS LETTERS, v.36, no.2, pp.148 - 150
Indexed
SCIE
SCOPUS
Journal Title
OPTICS LETTERS
Volume
36
Number
2
Start Page
148
End Page
150
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/113265
DOI
10.1364/OL.36.000148
ISSN
0146-9592
Abstract
We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon. (C) 2011 Optical Society of America
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