Validation of a Real-Time RT-PCR Method to Quantify Newcastle Disease Virus (NDV) Titer and Comparison with Other Quantifiable Methods
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Juno | - |
dc.contributor.author | Hong, Sung-Hwan | - |
dc.contributor.author | Kim, Ik-Hwan | - |
dc.date.accessioned | 2021-09-07T16:22:34Z | - |
dc.date.available | 2021-09-07T16:22:34Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-01 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/113313 | - |
dc.description.abstract | A method for the rapid detection and quantification of Newcastle disease virus (NDV) produced in an animal cell culture-based production system was developed to enhance the speed of the NDV vaccine manufacturing process. A SYBR Green I-based real-time RT-PCR was designed with a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The validation results satisfied the predetermined acceptance criteria. The validated method was used to quantify virus samples produced in an animal cell culture-based production system. The method was able to quantify the NDV samples from mid- or late-production phases, but not effective on samples from the early-production phase. For comparison with other quantifiable methods, immunoblotting, plaque assay, and tissue culture infectious dose 50 (TCID50) assay were also performed with the NDV samples. The results demonstrated that the real-time RT-PCR method is suitable for the rapid quantification of virus particles produced in an animal cell-culture-based production system irrespective of viral infectivity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | REVERSE-TRANSCRIPTION PCR | - |
dc.subject | AVIAN INFLUENZA | - |
dc.subject | FUSION PROTEIN | - |
dc.subject | CLEAVAGE SITE | - |
dc.subject | VACCINE | - |
dc.subject | ASSAY | - |
dc.subject | PATHOGENESIS | - |
dc.subject | VIRULENCE | - |
dc.subject | RNA | - |
dc.title | Validation of a Real-Time RT-PCR Method to Quantify Newcastle Disease Virus (NDV) Titer and Comparison with Other Quantifiable Methods | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Ik-Hwan | - |
dc.identifier.doi | 10.4014/jmb.1006.06006 | - |
dc.identifier.scopusid | 2-s2.0-79251621001 | - |
dc.identifier.wosid | 000286797800014 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.1, pp.100 - 108 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 21 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 100 | - |
dc.citation.endPage | 108 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001521594 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | REVERSE-TRANSCRIPTION PCR | - |
dc.subject.keywordPlus | AVIAN INFLUENZA | - |
dc.subject.keywordPlus | FUSION PROTEIN | - |
dc.subject.keywordPlus | CLEAVAGE SITE | - |
dc.subject.keywordPlus | VACCINE | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | VIRULENCE | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordAuthor | Method comparison | - |
dc.subject.keywordAuthor | method validation | - |
dc.subject.keywordAuthor | Newcastle disease virus (NDV) | - |
dc.subject.keywordAuthor | real-time RT-PCR (RRT-PCR) | - |
dc.subject.keywordAuthor | virus quantification | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.