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Phenotypic and Physiological Changes in Acinetobacter sp. Strain DR1 with Exogenous Plasmid

Authors
Park, JungsoonPark, Woojun
Issue Date
1월-2011
Publisher
SPRINGER
Citation
CURRENT MICROBIOLOGY, v.62, no.1, pp.249 - 254
Indexed
SCIE
SCOPUS
Journal Title
CURRENT MICROBIOLOGY
Volume
62
Number
1
Start Page
249
End Page
254
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/113318
DOI
10.1007/s00284-010-9698-y
ISSN
0343-8651
Abstract
The genus Acinetobacter has been recognized to take up exogenous DNA from the environment. In this study, we conducted natural transformation with a novel diesel-degrading Acinetobacter sp. strain, designated strain DR1, using the broad host range plasmid pRK415. Many factors, including temperature, quantities of DNA, and aeration have proven critically important for efficient natural transformation. Interestingly, the Acinetobacter sp. strain DR1 (pRK415) differed both phenotypically and physiologically from the wild-type strain in several regards, including motility, biofilm formation ability, and responses to oxidative stress: the transformed cells were rendered more sensitive to hydrogen peroxide and cumene hydroperoxide, and their motilities and biofilm formation activity were also attenuated. Our data demonstrated that caution should be exercised when conducting genetic manipulation with plasmids, due to the possibility that phenotypic and physiological changes in the host might occur along with the uptake of plasmids.
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Park, Woo jun
생명과학대학 (환경생태공학부)
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