Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process
- Authors
- Lee, Jong Ho; Kim, Sung Bong; Kang, Seong Woo; Song, Yoon Seok; Park, Chulhwan; Han, Sung Ok; Kim, Seung Wook
- Issue Date
- 1월-2011
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Biodiesel; Lipase; Initial reaction rate; Optimization; Supercritical carbon dioxide process
- Citation
- BIORESOURCE TECHNOLOGY, v.102, no.2, pp.2105 - 2108
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 102
- Number
- 2
- Start Page
- 2105
- End Page
- 2108
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113372
- DOI
- 10.1016/j.biortech.2010.08.034
- ISSN
- 0960-8524
- Abstract
- In this study, various factors, such as temperature, pressure, agitation speed, water content, and the concentration and ratio of immobilized ROL and CRL were investigated for the efficient enzymatic production of biodiesel using a supercritical carbon dioxide process. Furthermore, a stepwise reaction method for the maintenance of immobilized lipase activity was optimized. Optimal conditions for biodiesel production were determined to be as follows: 130 bar pressure, 45 degrees C temperature, 250 rpm agitation speed, 10% water content, and 20% immobilized ROL and CRL (1:1). When batch process was performed under optimal conditions, the biodiesel conversion yield was 99.13% at 3 h. Biodiesel conversion yield was 99.99% at 2 h when 90 mmol methanol was used in a stepwise reaction. Moreover, the conversion yield of biodiesel produced by the repeated recycling of immobilized lipase in the stepwise reactions was 85% after 20 reuses. (C) 2010 Elsevier Ltd. All rights reserved.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
- College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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