Barley DNA-binding methionine aminopeptidase, which changes the localization from the nucleus to the cytoplasm by low temperature, is involved in freezing tolerance
- Authors
- Jeong, Hee-Jeong; Shin, Jeong Sheop; Ok, Sung Han
- Issue Date
- 1월-2011
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Methionine aminopeptidase; Low-temperature; Freezing tolerance; Barley
- Citation
- PLANT SCIENCE, v.180, no.1, pp.53 - 60
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLANT SCIENCE
- Volume
- 180
- Number
- 1
- Start Page
- 53
- End Page
- 60
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113426
- DOI
- 10.1016/j.plantsci.2010.09.004
- ISSN
- 0168-9452
- Abstract
- The polymerase chain reaction-based Mirror Orientation Selection (MOS) method was used to isolate low temperature-induced genes from cold-treated winter barley (Hordeum vulgare L cv. Dongbori). MOS screening identified a novel methionine (Met) aminopeptidase (MAP) designated as HvMAP. The deduced HvMAP protein was determined to possess an aminopeptidase domain and a nuclear localization signal. An in vitro enzyme assay using recombinant HvMAP protein demonstrated MAP activity. The expression of this gene was induced by low temperature and abscisic acid treatment, and overexpression of this gene conferred stronger freezing tolerance to Arabidopsis transgenic plants as compared to wild-type plants. Interestingly, low temperature treatment changed the localization of HvMAP from the nucleus to the cytoplasm. These findings suggest that HvMAP is a novel MAP that functions in freezing tolerance by facilitating protein maturation. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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