ToF-SIMS and PCA of surface-immobilized antibodies with different orientations
DC Field | Value | Language |
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dc.contributor.author | Park, Ji-Won | - |
dc.contributor.author | Cho, Il-Hoon | - |
dc.contributor.author | Moon, Dae Won | - |
dc.contributor.author | Paek, Se-Hwan | - |
dc.contributor.author | Lee, Tae Geol | - |
dc.date.accessioned | 2021-09-07T16:44:09Z | - |
dc.date.available | 2021-09-07T16:44:09Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011-01 | - |
dc.identifier.issn | 0142-2421 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/113429 | - |
dc.description.abstract | A number of studies have been done on the orientation of surface-immobilized antibodies since it plays a significant role in the performance of immunoassays. Here, we present a new study by which the orientation of differently immobilized antibodies was directly probed by using time-of-flight secondary ion mass spectrometry (ToF-SIMS) and principal component analysis (PCA). For greater control over the orientations of intact IgG and F(ab')(2) antibody fragment, they were either site-directly biotinylated at the hinge region or randomly biotinylated at the amino groups, and were then immobilized onto a streptavidin-terminated surface. According to the PCA results from ToF-SIMS spectra, site-directly and randomly biotinylated IgG became 'end-on' oriented (Fc is closer to the surface) at a gradual rate as immobilization concentration increased, while site-directly biotinylated IgG became oriented at a slightly faster rate. Furthermore, site-directly biotinylated F(ab')(2) quickly became 'end-on' oriented even at a low concentration of 1 mu g/ml as immobilization concentration increased, whereas randomly biotinylated F(ab')(2) was not oriented at all over any concentration. Our results show that a ToF-SIMS partnership with multivariate analysis is useful for interpreting protein orientations in terms of surface amino acid profiles. Copyright (C) 2010 John Wiley & Sons, Ltd. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | ION MASS-SPECTROMETRY | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | ADSORBED PROTEIN FILMS | - |
dc.subject | MULTIVARIATE-ANALYSIS | - |
dc.subject | IMMUNOSENSOR | - |
dc.title | ToF-SIMS and PCA of surface-immobilized antibodies with different orientations | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paek, Se-Hwan | - |
dc.identifier.doi | 10.1002/sia.3440 | - |
dc.identifier.wosid | 000287669500072 | - |
dc.identifier.bibliographicCitation | SURFACE AND INTERFACE ANALYSIS, v.43, no.1-2, pp.285 - 289 | - |
dc.relation.isPartOf | SURFACE AND INTERFACE ANALYSIS | - |
dc.citation.title | SURFACE AND INTERFACE ANALYSIS | - |
dc.citation.volume | 43 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 285 | - |
dc.citation.endPage | 289 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.subject.keywordPlus | ION MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | ADSORBED PROTEIN FILMS | - |
dc.subject.keywordPlus | MULTIVARIATE-ANALYSIS | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordAuthor | ToF-SIMS | - |
dc.subject.keywordAuthor | principal component analysis | - |
dc.subject.keywordAuthor | antibody | - |
dc.subject.keywordAuthor | orientation | - |
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