Production of 3,6-anhydro-L-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40
- Authors
- Yun, Eun Ju; Shin, Min Hye; Yoon, Jeong-Jun; Kim, Yong Jin; Choi, In-Geol; Kim, Kyoung Heon
- Issue Date
- 1월-2011
- Publisher
- ELSEVIER SCI LTD
- Keywords
- 3,6-Anhydro-L-galactose; Saccharophagus degradans; Agarase; Red macroalgae; Gas chromatography-mass spectrometry
- Citation
- PROCESS BIOCHEMISTRY, v.46, no.1, pp.88 - 93
- Indexed
- SCIE
SCOPUS
- Journal Title
- PROCESS BIOCHEMISTRY
- Volume
- 46
- Number
- 1
- Start Page
- 88
- End Page
- 93
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113442
- DOI
- 10.1016/j.procbio.2010.07.019
- ISSN
- 1359-5113
- Abstract
- Saccharophagus degradans 2-40 is capable of hydrolyzing agarose, a red macroalgae-derived polymer, into D-galactose and 3,6-anhydro-L-galactose (L-AHG). Its agarase system is receiving much attention because it can be used to produce fermentable sugar from agarose. L-AHG is commercially unavailable and is considered a rare sugar with a high value. In this study, cells grown on agarose, agar or red macroalgae biomass were found to have a significantly higher agarase activity and AHG-generating activity than those grown on glucose or galactose. From agar-grown cells, both the volumetric activities of agarases and AHG generation in the cell-free lysate were much higher than in the extracellular fraction. Based on the analyses of the enzyme reaction products, from the reaction with the crude enzymes from cell-free lysate, neoagarobiose with a degree of polymerization (DP) 2 appeared to be the only major product in the initial reaction period, but sugars with DPs 2, 4 and 6 were found to be all predominantly produced by the extracellular enzymes in the initial reaction period. Quantitative analysis of AHG using gas chromatography-mass spectrometry with a derivatization step was also found to be highly reproducible and reliable. These results will be useful for producing L-AHG as a rare sugar to investigate its metabolic fate and commercial utilization. (C) 2010 Elsevier Ltd. All rights reserved.
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