한국에서의 2009 신종 인플루엔자 A의 임상양상과 다양한 진단 방법들의 비교Clinical Characteristics and Comparison of the Various Methods Used for the Diagnosis of the New Influenza A Pandemic in Korea
- Other Titles
- Clinical Characteristics and Comparison of the Various Methods Used for the Diagnosis of the New Influenza A Pandemic in Korea
- Authors
- 권민정; 이창규; 노경호; 남명현; 윤수영; 임채승; 조윤정; 김영기; 이갑노
- Issue Date
- 2011
- Publisher
- 대한진단검사의학회
- Keywords
- New influenza; Rapid antigen test; Culture; Real-time RT-PCR
- Citation
- Laboratory Medicine Online, v.1, no.1, pp.26 - 34
- Indexed
- KCI
OTHER
- Journal Title
- Laboratory Medicine Online
- Volume
- 1
- Number
- 1
- Start Page
- 26
- End Page
- 34
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113453
- ISSN
- 2093-6338
- Abstract
- Background: Laboratory diagnosis of new influenza A (H1N1) is crucial for managing patients and establishing control and prevention measures.
We compared the diagnostic accuracies of the real time RT-PCR (rRT-PCR) test recommended for the confirmation of the new flu and the viral culture method used conventionally for viral disease with that of the rapid antigen test (RAT).
Methods: We performed RAT, R-mix culture, and real-time PCR by using 861 respiratory samples collected from December 2009 to January 2010and evaluated the abilities of these methods to detect new influenza A. The relationship among the positive rates of RAT, grades of culture, and the cycle threshold (Ct) values of rRT-PCR was also evaluated.
Results: Of the 861 patients, 308 (35.8%) were diagnosed with new influenza A. The sensitivities, specificities, positive predictive values, and negative predictive values of the tests were respectively as follows: 59.7%, 99.5%, 98.4%, and 81.6% for RAT; 93.2%, 100%, 100%, and 96.3% for R-mix culture; and 95.8%, 100%, 100%, and 97.7% for rRT-PCR. Samples with weak positive grade in culture and those with Ct values of 30–37 in rRT-PCR showed positivities as low as 25.3% and 2.3% in RAT, respectively. The hospitalization rate and death rate of the confirmed patients were 3.2% and 0.3%, respectively, and gastrointestinal symptoms were observed in 7.2% of the patients.
Conclusions: R-mix culture and rRT-PCR tests showed excellent reliability in the diagnosis of new influenza A and could be very useful, especially for samples with low viral load.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
- College of Medicine > Department of Medical Science > 1. Journal Articles
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