Barium Stimulates the Expression of Osteogenic Genes in Human Mesenchymal Stem Cells into Osteoblasts in vitro
DC Field | Value | Language |
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dc.contributor.author | 안기훈 | - |
dc.contributor.author | So Eun Jung | - |
dc.contributor.author | 이경욱 | - |
dc.contributor.author | Hyun Tae Park | - |
dc.contributor.author | Jung Ho Shin | - |
dc.contributor.author | Young Tae Kim | - |
dc.contributor.author | Jun Young Hur | - |
dc.contributor.author | 김선행 | - |
dc.contributor.author | 김탁 | - |
dc.date.accessioned | 2021-09-07T17:12:46Z | - |
dc.date.available | 2021-09-07T17:12:46Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 2288-6478 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/113569 | - |
dc.description.abstract | Objectives: The purpose of this study was to determine the effect of barium on gene expression in differentiation of human mesenchymal stem cells into osteoblasts in vitro. Methods: Human bone marrow stem cells were cultured for 0∼14 days in osteogenic differentiation medium with strontium chloride (SrCl₂) and barium chloride (BaCl₂). Alkaline phosphatase (ALP) activity staining was the method selected for measuring osteoblast differentiation. Total ribonucleic acid (RNA) was extracted after 1, 3, 7, and 14 days, and analysis of runt-related transcription factor 2/core-binding factor alpha 1 (Runx2/Cbfa1), bone morphogenetic protein-2 (BMP-2), and bone sialoprotein (BSP) gene expression was performed by real-time reverse transcriptase (RT)-polymerase chain reaction (PCR). Results: Barium and strontium had a superior enhancing effect on cell proliferation when compared to cells cultured in media without strontium or barium. BaCl2 produced a 2-fold increase in the expression of Runx2/Cbfa1 at 14 days. SrCl₂(0.1∼0.3 mM) produced a 2-fold increase in the expression of Runx2/Cbfa1 at 14 days. Barium produced a 1.5-fold increase in the expression of BMP-2 on days 1 or 3. Expression of BSP was increased 1.5∼1.7- and 2-fold on days 1 and 14 by barium and strontium, respectively. Conclusion: Barium-like strontium is considered one of the important factors in inducing mesenchymal stem cells to differentiate into osteoblasts with further enhancement on bone formation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | 대한폐경학회 | - |
dc.title | Barium Stimulates the Expression of Osteogenic Genes in Human Mesenchymal Stem Cells into Osteoblasts in vitro | - |
dc.title.alternative | Barium Stimulates the Expression of Osteogenic Genes in Human Mesenchymal Stem Cells into Osteoblasts in vitro | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 이경욱 | - |
dc.contributor.affiliatedAuthor | 김선행 | - |
dc.contributor.affiliatedAuthor | 김탁 | - |
dc.identifier.bibliographicCitation | 대한폐경학회지, v.17, no.2, pp.81 - 87 | - |
dc.relation.isPartOf | 대한폐경학회지 | - |
dc.citation.title | 대한폐경학회지 | - |
dc.citation.volume | 17 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 81 | - |
dc.citation.endPage | 87 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART001675775 | - |
dc.description.journalClass | 2 | - |
dc.description.journalRegisteredClass | kci | - |
dc.description.journalRegisteredClass | other | - |
dc.subject.keywordAuthor | Barium | - |
dc.subject.keywordAuthor | Gene expression | - |
dc.subject.keywordAuthor | Osteoblast | - |
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