Aptamers-on-nanofiber as a novel hybrid capturing moiety
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ji Hoon | - |
dc.contributor.author | Hwang, Ee Taek | - |
dc.contributor.author | Kang, Kyung-kyu | - |
dc.contributor.author | Tatavarty, Rameshwar | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-07T21:23:56Z | - |
dc.date.available | 2021-09-07T21:23:56Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0959-9428 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/114913 | - |
dc.description.abstract | In this study, we present a new bio-inspired hybrid capturing moiety, aptamers on a polymeric polystyrene-poly(styrene-co-maleic anhydride) nanofiber, for the purification of target protein. These novel hybrid composites were successfully fabricated by immobilizing aptamers on nanofibers synthesized by electrospinning. The advantageous characteristics of nanofibers, such as a very large surface to volume ratio and flexible modification of the surface, contributed to the greatly improved surface coverage of aptamers, resulting in the highly increased capturing capacity of the target proteins. As a result, this increased capturing capacity led to 85% of recovery yield in the protein purification. In addition to easy preparation and cost effectiveness of this system, these novel aptamers-on-nanofiber composites showed good stability with no conspicuous reduction in the purification efficiency, even after repeated use for seven months. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | AFFINITY-CHROMATOGRAPHY | - |
dc.subject | RNA APTAMER | - |
dc.subject | DNA | - |
dc.subject | SEPARATION | - |
dc.subject | EXPRESSION | - |
dc.subject | ADENOSINE | - |
dc.subject | BIND | - |
dc.title | Aptamers-on-nanofiber as a novel hybrid capturing moiety | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Tatavarty, Rameshwar | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1039/c1jm13558a | - |
dc.identifier.scopusid | 2-s2.0-82455167156 | - |
dc.identifier.wosid | 000297559400002 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MATERIALS CHEMISTRY, v.21, no.48, pp.19203 - 19206 | - |
dc.relation.isPartOf | JOURNAL OF MATERIALS CHEMISTRY | - |
dc.citation.title | JOURNAL OF MATERIALS CHEMISTRY | - |
dc.citation.volume | 21 | - |
dc.citation.number | 48 | - |
dc.citation.startPage | 19203 | - |
dc.citation.endPage | 19206 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | AFFINITY-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | RNA APTAMER | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ADENOSINE | - |
dc.subject.keywordPlus | BIND | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.