Minimum-step immuno-analysis based on continuous recycling of the capture antibody
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Hyun-Kyu | - |
dc.contributor.author | Seo, Sung-Min | - |
dc.contributor.author | Cho, Il-Hoon | - |
dc.contributor.author | Paek, Sung-Ho | - |
dc.contributor.author | Kim, Dong-Hyung | - |
dc.contributor.author | Paek, Se-Hwan | - |
dc.date.accessioned | 2021-09-07T21:45:25Z | - |
dc.date.available | 2021-09-07T21:45:25Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/114984 | - |
dc.description.abstract | Most immuno-analytical systems employ antibodies that do not readily dissociate upon binding to its partner antigen (i.e., target analyte; alpha 2-macroglobulin as a model) and, thus, either need to be disposed of after one-time use or be reused after binding has been reset. To achieve a minimum-step analysis, an antibody that is capable of rapidly reversible binding with high affinity to an antigen was investigated in this study. This antibody was immobilized on the surface of a label-free sensor, which was combined with microfluidic channels, to demonstrate its applicability. The antibody was successively reused without a regeneration step under physiological conditions, offered specific analysis in the serum medium, and detected the analyte at concentrations as low as 0.1 ng mL(-1), which could further be enhanced by 100-fold. The sensor response reached 95% equilibrium after 8.3 and 14.9 min in average on each dose level for the concentration increase and decrease, respectively. The dynamic range covered a 5 logarithmic analyte concentration. Since the sampling size was in the nanolitre to millilitre range per day under the conditions used and the sensor may retain a long shelf-life, it could potentially be used in a clinical setting for long-term, on-line monitoring of diseases. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | PROTEIN INTERACTIONS | - |
dc.subject | CELL | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | CHROMATOGRAPHY | - |
dc.subject | IMMUNOSENSORS | - |
dc.subject | RECOGNITION | - |
dc.subject | MODEL | - |
dc.subject | ASSAY | - |
dc.title | Minimum-step immuno-analysis based on continuous recycling of the capture antibody | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paek, Se-Hwan | - |
dc.identifier.doi | 10.1039/c0an00811g | - |
dc.identifier.scopusid | 2-s2.0-79952613759 | - |
dc.identifier.wosid | 000288327600015 | - |
dc.identifier.bibliographicCitation | ANALYST, v.136, no.7, pp.1374 - 1379 | - |
dc.relation.isPartOf | ANALYST | - |
dc.citation.title | ANALYST | - |
dc.citation.volume | 136 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1374 | - |
dc.citation.endPage | 1379 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | PROTEIN INTERACTIONS | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
dc.subject.keywordPlus | IMMUNOSENSORS | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | ASSAY | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.