A zinc finger protein Tsip1 controls Cucumber mosaic virus infection by interacting with the replication complex on vacuolar membranes of the tobacco plant
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Huh, Sung Un | - |
dc.contributor.author | Kim, Min Jung | - |
dc.contributor.author | Ham, Byung-Kook | - |
dc.contributor.author | Paek, Kyung-Hee | - |
dc.date.accessioned | 2021-09-07T21:58:26Z | - |
dc.date.available | 2021-09-07T21:58:26Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0028-646X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115057 | - |
dc.description.abstract | In Cucumber mosaic virus (CMV) RNA replication, replicase-associated protein CMV 1a and RNA-dependent RNA polymerase protein CMV 2a are essential for formation of an active virus replicase complex on vacuolar membranes. To identify plant host factors involved in CMV replication, a yeast two-hybrid system was used with CMV 1a protein as bait. One of the candidate genes encoded Tsi1-interacting protein 1 (Tsip1), a zinc (Zn) finger protein. Tsip1 strongly interacted with CMV 2a protein, too. Formation of a Tsip1 complex involving CMV 1a or CMV 2a was confirmed in vitro and in planta. When 35S::Tsip1 tobacco (Nicotiana tabacum) plants were inoculated with CMV-Kor, disease symptom development was delayed and the accumulation of CMV RNAs and coat protein was decreased in both the infected local leaves and the uninfected upper leaves, compared with the wild type, whereas Tsip1-RNAi plants showed modestly but consistently increased CMV susceptibility. In a CMV replication assay, CMV RNA concentrations were reduced in the 35S::Tsip1 transgenic protoplasts compared with wild-type (WT) protoplasts. These results indicate that Tsip1 might directly control CMV multiplication in tobacco plants by formation of a complex with CMV 1a and CMV 2a. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | DEPENDENT RNA-POLYMERASE | - |
dc.subject | TURNIP CRINKLE VIRUS | - |
dc.subject | HEAT-SHOCK RESPONSE | - |
dc.subject | MOLECULAR CHAPERONE | - |
dc.subject | HSP70 CHAPERONES | - |
dc.subject | CAPSID PROTEIN | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | CELLS | - |
dc.subject | HOST | - |
dc.subject | DNAJ | - |
dc.title | A zinc finger protein Tsip1 controls Cucumber mosaic virus infection by interacting with the replication complex on vacuolar membranes of the tobacco plant | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paek, Kyung-Hee | - |
dc.identifier.doi | 10.1111/j.1469-8137.2011.03717.x | - |
dc.identifier.scopusid | 2-s2.0-79960556516 | - |
dc.identifier.wosid | 000292924600014 | - |
dc.identifier.bibliographicCitation | NEW PHYTOLOGIST, v.191, no.3, pp.746 - 762 | - |
dc.relation.isPartOf | NEW PHYTOLOGIST | - |
dc.citation.title | NEW PHYTOLOGIST | - |
dc.citation.volume | 191 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 746 | - |
dc.citation.endPage | 762 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | DEPENDENT RNA-POLYMERASE | - |
dc.subject.keywordPlus | TURNIP CRINKLE VIRUS | - |
dc.subject.keywordPlus | HEAT-SHOCK RESPONSE | - |
dc.subject.keywordPlus | MOLECULAR CHAPERONE | - |
dc.subject.keywordPlus | HSP70 CHAPERONES | - |
dc.subject.keywordPlus | CAPSID PROTEIN | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | HOST | - |
dc.subject.keywordPlus | DNAJ | - |
dc.subject.keywordAuthor | CMV 1a protein | - |
dc.subject.keywordAuthor | CMV 2a protein | - |
dc.subject.keywordAuthor | Cucumber mosaic virus | - |
dc.subject.keywordAuthor | systemic movement | - |
dc.subject.keywordAuthor | Tsip1 | - |
dc.subject.keywordAuthor | yeast two-hybrid | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.