Low-dose UVB irradiation stimulates matrix metalloproteinase-1 expression via a BLT2-linked pathway in HaCaT cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Cheolmin | - |
dc.contributor.author | Ryu, Ho-Cheol | - |
dc.contributor.author | Kim, Jae-Hong | - |
dc.date.accessioned | 2021-09-07T22:04:11Z | - |
dc.date.available | 2021-09-07T22:04:11Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-12-31 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115089 | - |
dc.description.abstract | Skin exposure to low-dose ultraviolet B (UVB) light up-regulates the expression of matrix metalloproteinase-1 (MMP-1), thus contributing to premature skin aging (photo-aging). Although cyclooxygenase-2 (COX-2) and its product, prostaglandin E-2 (PGE(2)), have been associated with UVB-induced signaling to MMP expression, very little are known about the roles of lip-oxygenases and their products, especially leukotriene B-4 (LTB4) and 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE), in MMP-1 expression in skin keratinocytes. In the present study, we demonstrate that BLT2, a cell surface receptor for LTB4 and 12(S)-HETE, plays a critical role in UVB-mediated MMP-1 upregulation in human HaCaT keratinocytes. Moreover, our results demonstrated that BLT2-mediated MMP-1 upregulation occurs through a signaling pathway dependent on reactive oxygen species (ROS) production and the subsequent stimulation of ERK. Blockage of BLT2 via siRNA knockdown or with the BLT2-antagonist LY255283 completely abolished the up-regulated expression of MMP-1 induced by low-dose UVB irradiation. Finally, when HaCaT cells were transiently transfected with a BLT2 expression plasmid, MMP-1 expression was significantly enhanced, along with ERK phosphorylation, suggesting that BLT2 overexpression alone is sufficient for MMP-1 up-regulation. Together, our results suggest that the BLT2-ROS-ERK-linked cascade is a novel signaling mechanism for MMP-1 upregulation in low-dose UVB- irradiated keratinocytes and thus potentially contributes to photo-aging. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | HUMAN DERMAL FIBROBLASTS | - |
dc.subject | SIGNAL-REGULATED KINASE | - |
dc.subject | SKIN IN-VIVO | - |
dc.subject | HUMAN KERATINOCYTES | - |
dc.subject | LEUKOTRIENE B-4 | - |
dc.subject | ULTRAVIOLET-RADIATION | - |
dc.subject | LINKED CASCADE | - |
dc.subject | INDUCED MMP-1 | - |
dc.subject | ACTIVATION | - |
dc.subject | RECEPTOR | - |
dc.title | Low-dose UVB irradiation stimulates matrix metalloproteinase-1 expression via a BLT2-linked pathway in HaCaT cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jae-Hong | - |
dc.identifier.doi | 10.3858/emm.2010.42.12.086 | - |
dc.identifier.scopusid | 2-s2.0-78650832954 | - |
dc.identifier.wosid | 000285813300005 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.42, no.12, pp.833 - 841 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 42 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 833 | - |
dc.citation.endPage | 841 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001504024 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | HUMAN DERMAL FIBROBLASTS | - |
dc.subject.keywordPlus | SIGNAL-REGULATED KINASE | - |
dc.subject.keywordPlus | SKIN IN-VIVO | - |
dc.subject.keywordPlus | HUMAN KERATINOCYTES | - |
dc.subject.keywordPlus | LEUKOTRIENE B-4 | - |
dc.subject.keywordPlus | ULTRAVIOLET-RADIATION | - |
dc.subject.keywordPlus | LINKED CASCADE | - |
dc.subject.keywordPlus | INDUCED MMP-1 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordAuthor | 12-hydroxy-5,8,10,14-eicosatetraenoic acid | - |
dc.subject.keywordAuthor | leukotriene B-4 | - |
dc.subject.keywordAuthor | LTB(4)R2 protein, human | - |
dc.subject.keywordAuthor | matrix metalloproteinase 1 | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | skin aging | - |
dc.subject.keywordAuthor | ultraviolet rays | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.