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Kidney-Derived Mesenchymal Stromal Cells Modulate Dendritic Cell Function to Suppress Alloimmune Responses and Delay Allograft Rejection

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dc.contributor.authorHuang, Yanfei-
dc.contributor.authorChen, Ping-
dc.contributor.authorZhang, Cassie B.-
dc.contributor.authorKo, Gang Jee-
dc.contributor.authorRuiz, Miriam-
dc.contributor.authorFiorina, Paolo-
dc.contributor.authorHussain, Mehboob A.-
dc.contributor.authorWasowska, Barbara A.-
dc.contributor.authorRabb, Hamid-
dc.contributor.authorWomer, Karl L.-
dc.date.accessioned2021-09-07T22:07:08Z-
dc.date.available2021-09-07T22:07:08Z-
dc.date.issued2010-12-27-
dc.identifier.issn0041-1337-
dc.identifier.issn1534-6080-
dc.identifier.urihttps://scholar.korea.ac.kr/handle/2021.sw.korea/115095-
dc.description.abstractBackground. Mesenchymal stromal cells (MSCs) are multipotent cells with immunoregulatory capacity that are present in most adult organs. We previously demonstrated that co-culture of C57BL/6 kidney-derived MSCs (KSCs) in syngeneic bone marrow-derived dendritic cell (DC) culture induced a DC phenotype (KSC-DC) with reduced major histocompatibility complex (MHC) class II/increased CD80 expression and ability to suppress T-cell responses. Methods. To study their effects on allogeneic DCs, C57BL/6 KSCs were added to incipient BALB/c DC culture, with surface expression of MHC class II/CD80 measured by fluorescence-activated cell sorting. The ability to stimulate T-cell responses was then assessed in an allogeneic mixed leukocyte response. Next, we isolated either BALB/c (donor) or C57BL/6 (recipient) KSC-DCs from co-culture and measured the tempo of rejection after cotransplantation with islet grafts in a mouse model of islet transplantation. Finally, we measured the effects of KSC-DC stimulation on B-cell proliferation and IgM/IgG production in allogeneic cultures. Results. C57BL/6 KSCs induced a BALB/c DC phenotype with significantly decreased MHC class II, increased CD80 expression, and decreased T-cell stimulatory capacity in the mixed leukocyte response (P < 0.01 vs. control). Cotransplantation of donor (BALB/c) but not recipient (C57BL/6) KSC-DCs resulted in significant delay of rejection after islet transplantation (P < 0.01 vs. control). Finally, stimulation by KSC-DCs resulted in significantly reduced B-cell proliferation and antibody production in allogeneic culture (P < 0.01 vs. control). Conclusions. Our results highlight an important mechanism of MSC-based immunotherapy and its potential for use in clinical transplantation as prevention of rejection and possibly sensitization.-
dc.format.extent5-
dc.language영어-
dc.language.isoENG-
dc.publisherLIPPINCOTT WILLIAMS & WILKINS-
dc.titleKidney-Derived Mesenchymal Stromal Cells Modulate Dendritic Cell Function to Suppress Alloimmune Responses and Delay Allograft Rejection-
dc.typeArticle-
dc.publisher.location미국-
dc.identifier.doi10.1097/TP.0b013e3181fdd9eb-
dc.identifier.scopusid2-s2.0-78650842941-
dc.identifier.wosid000285377100011-
dc.identifier.bibliographicCitationTRANSPLANTATION, v.90, no.12, pp 1307 - 1311-
dc.citation.titleTRANSPLANTATION-
dc.citation.volume90-
dc.citation.number12-
dc.citation.startPage1307-
dc.citation.endPage1311-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClasssci-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaImmunology-
dc.relation.journalResearchAreaSurgery-
dc.relation.journalResearchAreaTransplantation-
dc.relation.journalWebOfScienceCategoryImmunology-
dc.relation.journalWebOfScienceCategorySurgery-
dc.relation.journalWebOfScienceCategoryTransplantation-
dc.subject.keywordPlusHEMATOPOIETIC STEM-CELLS-
dc.subject.keywordPlusBONE-MARROW-
dc.subject.keywordPlusTRANSPLANTATION-
dc.subject.keywordPlusPROLIFERATION-
dc.subject.keywordPlusSURVIVAL-
dc.subject.keywordPlusGVHD-
dc.subject.keywordAuthorDendritic cells-
dc.subject.keywordAuthorAntigen-presenting cells-
dc.subject.keywordAuthorIslet transplantation-
dc.subject.keywordAuthorMesenchymal stem cells-
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