Pep-1 peptide-conjugated elastic liposomal formulation of taxifolin glycoside for the treatment of atopic dermatitis in NC/Nga mice
- Authors
- Kang, Myung Joo; Eum, Jae Yoon; Park, Sang Han; Kang, Mean Hyung; Park, Kwan Hee; Choi, Sun Eun; Lee, Min Won; Kang, Kyung Ho; Oh, Chil Hwan; Choi, Young Wook
- Issue Date
- 15-12월-2010
- Publisher
- ELSEVIER
- Keywords
- Taxifolin glycoside; Elastic liposomes; Pep-1 peptide; Atopic dermatitis; NC/Nga mice
- Citation
- INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.402, no.1-2, pp.198 - 204
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF PHARMACEUTICS
- Volume
- 402
- Number
- 1-2
- Start Page
- 198
- End Page
- 204
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/115120
- DOI
- 10.1016/j.ijpharm.2010.09.030
- ISSN
- 0378-5173
- Abstract
- In order to develop topical preparations of taxifolin glycoside (TXG) for the treatment of atopic dermatitis (AD), formulations of Pep-1 peptide-conjugated elastic liposomes (Pep1-EL) were examined for their in vitro skin permeation profile and in vivo therapeutic efficacy. TXG-loaded Pep1-EL - a nanovesicle consisting of phosphatidylcholine, Tween 80, N-[4-(p-maleimidophenyl)butyryll-phosphatidylethanolamine (MPB-PE), and Pep-1 peptide - is 130 nm in size, and has a zeta potential of 25 mV and a deformability index value of 60. Here, we examined the skin permeability of several topical preparations using a Franz diffusion cell mounted with depilated mouse skin and found that formulations of Pep1-EL exhibited superior absorption when compared to aqueous solution. EL or Pep-1 peptide-admixed EL formulations. Both transepidermal water loss and skin surface hydration were also measured using AD-induced NC/Nga mice, and the TXG-loaded Pep1-EL treatment group displayed a significantly expedited recovery in skin barrier function when compared to the controls treated with a TXG aqueous solution (p < 0.05). AD-associated immune responses - including serum interleukine-4, immunoglobulin E. and interferongamma - were also regulated by topical application of TXG-loaded Pep1-EL In conclusion, the novel Pep1-EL formulation of TXG shows substantial promise in the treatment of AD as a result of its desirable skin delivery-promoting capability. (C) 2010 Elsevier B.V. All rights reserved.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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