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Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

Authors
Bang, J.Beuchat, L. R.Gu, M. B.Chang, H. -I.Ryu, J. -H.
Issue Date
12월-2010
Publisher
WILEY
Keywords
DNA chip; identification; randomly fragmented genomic DNA; Yersinia enterocolitica
Citation
LETTERS IN APPLIED MICROBIOLOGY, v.51, no.6, pp.665 - 670
Indexed
SCIE
SCOPUS
Journal Title
LETTERS IN APPLIED MICROBIOLOGY
Volume
51
Number
6
Start Page
665
End Page
670
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115141
DOI
10.1111/j.1472-765X.2010.02954.x
ISSN
0266-8254
Abstract
Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability. Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria. Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y. enterocolitica can detect Y. enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera. Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y. enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y. enterocolitica was verified.
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